Test characteristics of acridine orange, Gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid

E.I.G.B. De Brauwer; J.A. Jacobs; F.H.M. Nieman; C.A.M.V.A. Bruggeman; M. Drent

doi:10.1128/JCM.37.2.427-429.1999

Test characteristics of acridine orange, Gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid

E.I.G.B. De Brauwer, J.A. Jacobs^*, F.H.M. Nieman, C.A.M.V.A. Bruggeman, M. Drent

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.

For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grunwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.

Original language	English
Pages (from-to)	427-429
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	37
Issue number	2
DOIs	https://doi.org/10.1128/JCM.37.2.427-429.1999
Publication status	Published - 1 Jan 1999

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10.1128/JCM.37.2.427-429.1999

http://www.ncbi.nlm.nih.gov/entrez/utils/fref.fcgi?http://jcm.asm.org/cgi/pmidlookup?view=full&pmid=9889233

Cite this

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title = "Test characteristics of acridine orange, Gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid",

abstract = "Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grunwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.",

author = "{De Brauwer}, E.I.G.B. and J.A. Jacobs and F.H.M. Nieman and C.A.M.V.A. Bruggeman and M. Drent",

year = "1999",

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doi = "10.1128/JCM.37.2.427-429.1999",

language = "English",

volume = "37",

pages = "427--429",

journal = "Journal of Clinical Microbiology",

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publisher = "American Society for Microbiology",

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Test characteristics of acridine orange, Gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid. / De Brauwer, E.I.G.B.; Jacobs, J.A.; Nieman, F.H.M. et al.
In: Journal of Clinical Microbiology, Vol. 37, No. 2, 01.01.1999, p. 427-429.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Test characteristics of acridine orange, Gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid

AU - De Brauwer, E.I.G.B.

AU - Jacobs, J.A.

AU - Nieman, F.H.M.

AU - Bruggeman, C.A.M.V.A.

AU - Drent, M.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grunwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.

AB - Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grunwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.

U2 - 10.1128/JCM.37.2.427-429.1999

DO - 10.1128/JCM.37.2.427-429.1999

M3 - Article

C2 - 9889233

SN - 0095-1137

VL - 37

SP - 427

EP - 429

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -