Test characteristics of acridine orange, Gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid

E.I.G.B. De Brauwer, J.A. Jacobs, F.H.M. Nieman, C.A.M.V.A. Bruggeman, M. Drent

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.

For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grunwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.

Original languageEnglish
Pages (from-to)427-429
Number of pages3
JournalJournal of Clinical Microbiology
Volume37
Issue number2
DOIs
Publication statusPublished - 1 Jan 1999

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