@article{aa3374ac98a147a589b1dec2f2421dc5,
title = "Targeted Phosphoinositides Analysis Using High-Performance Ion Chromatography-Coupled Selected Reaction Monitoring Mass Spectrometry",
abstract = "Phosphoinositides are minor components of cell membranes, but play crucial roles in numerous signal transduction pathways. To obtain quantitative measures of phosphoinositides, sensitive, accurate, and comprehensive methods are needed. Here, we present a quantitative targeted ion chromatography-mass spectrometry-based workflow that separates phosphoinositide isomers and increases the quantitative accuracy of measured phosphoinositides. Besides testing different analytical characteristics such as extraction and separation efficiency, the reproducibility of the developed workflow was also investigated. The workflow was verified in resting and stimulated human platelets, fat cells, and rat hippocampal brain tissue, where the LOD and LOQ for phosphoinositides were at 312.5 and 625 fmol, respectively. The robustness of the workflow is shown with different applications that confirms its suitability to analyze multiple less-abundant phosphoinositides.",
keywords = "phosphoinositides, targeted lipidomics, ion chromatography, NECK-CANCER CELLS, RAT-BRAIN, INSULIN, QUANTIFICATION, LIPIDS, METABOLITES, 3-KINASE, HEAD",
author = "Cheung, {Hilaire Yam Fung} and Cristina Coman and Philipp Westhoff and Mailin Manke and Albert Sickmann and Oliver Borst and Meinrad Gawaz and Watson, {Steve P.} and Heemskerk, {Johan W. M.} and Robert Ahrends",
note = "Funding Information: This study was supported by the grants from the Deutsche Forschungsgemeinschaft and FWF Der Wissenschaftsfonds (P33298-B). The authors received further support from the Federal Ministry of Education and Research (BMBF) in the German Network Bioinformatics Infrastructure (de.NBI) initiative grant nos. 031L0108A and 031A534B. H.Y.F.C. is supported by the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 766118 (TAPAS) and is enrolled in a joint Ph.D. program of the Universities of Maastricht (The Netherlands) and University of Birmingham (U.K.). Funding Information: This study was supported by the grants from the Deutsche Forschungsgemeinschaft and FWF Der Wissenschaftsfonds (P33298-B). The authors received further support from the Federal Ministry of Education and Research (BMBF) in the German Network Bioinformatics Infrastructure (de.NBI) initiative grant nos. 031L0108A and 031A534B. H.Y.F.C. is supported by the European Union?s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 766118 (TAPAS) and is enrolled in a joint Ph.D. program of the Universities of Maastricht (The Netherlands) and University of Birmingham (U.K.). Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",
year = "2021",
month = jun,
day = "4",
doi = "10.1021/acs.jproteome.1c00017",
language = "English",
volume = "20",
pages = "3114--3123",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "6",
}