Abstract
Background
Brown adipose tissue research is in the focus in the field of endocrinology. We designed a dual-modal fluorescent/PET fatty acid based tracer on commercially available Bodipy-C-16, which can be synthesized to its corresponding triglyceride and which combines the benefits of fluorescent and PET imaging.
Methods
Bodipy-C-16 was coupled to 1,3-diolein resulting in Bodipy-triglyceride. Bodipy-C-16 and Bodipy-triglyceride compounds were radiolabeled with F-18 using an F-18/F-19 exchange reaction to yield a dual-modal imaging molecule. Uptake of radiolabeled and non-labeled Bodipy-C-16 and Bodipy-triglyceride was analyzed by fluorescence imaging and radioactive uptake in cultured adipocytes derived from human brown adipose tissue and white adipose tissue.
Results
Bodipy-C-16 and Bodipy-triglyceride were successfully radiolabeled and Bodipy-C-16 showed high shelf life and blood plasma stability (99% from 0-4 h). The uptake of Bodipy-C-16 increased over time in cultured adipocytes, which was further enhanced after beta-adrenergic stimulation with norepinephrine. The uptake of Bodipy-C-16 was inhibited by oleic acid and CD36 inhibitor sulfosuccinimidyl-oleate. The poor solubility of Bodipy-triglyceride did not allow stability or in vitro experiments.
Conclusion
The new developed dual modal fatty acid based tracers Bodipy-C-16 and Bodipy-triglyceride showed promising results to stimulate further in vivo evaluation and will help to understand brown adipose tissues role in whole body energy expenditure.
Original language | English |
---|---|
Article number | 0182297 |
Number of pages | 13 |
Journal | PLOS ONE |
Volume | 12 |
Issue number | 8 |
DOIs | |
Publication status | Published - 17 Aug 2017 |
Keywords
- FATTY-ACID UPTAKE
- POSITRON-EMISSION-TOMOGRAPHY
- MAGNETIC-RESONANCE
- ADULT HUMANS
- LIPOPROTEIN-LIPASE
- COLD-EXPOSURE
- BODIPY DYES
- TRANSPORT
- METABOLISM
- ADIPOCYTES