PURPOSE. To determine the feasibility of confocal profiling in measuring surface roughness and obtaining 3-dimensional reconstructions of mechanically dissected and femtosecond (fs)-laser photodisrupted endothelial lamellae. To determine the predictability of single-pass dissection of ultrathin endothelial lamellae using a novel motor-driven linear microkeratome.
METHODS. Thirty (n = 30) human corneas were harvested using a motor-driven linear microkeratome (n = 20); a hand-driven rotatory microkeratome (n = 6); and a 60-kHz fs laser (n = 4). Surface roughness was measured using an optical profiler operated in confocal microscopy mode followed by environmental scanning-electron-microscopy.
RESULTS. Mean surface roughness for the fs laser, motor-driven linear microkeratome, and hand-driven rotatory microkeratome measured 1.90 +/- 0.48 mu m, 1.06 +/- 0.42 mu m, and 0.93 +/- 0.25 mu m, respectively. Femtosecond photodisrupted lamellae were significantly rougher than mechanically dissected lamellae (P <0.001). Mean (+/- SD) cutting depth with the motor-driven linear microkeratome measured: 552 +/- 11 mu m (550-mu m head); 505 +/- 19 mu m (550-mu m head); 459 +/- 19 mu m (450-mu m head); and 392 +/- 20 mu m (400-mu m head).
CONCLUSIONS. Confocal microscopy allows quantitative surface roughness analysis and 3-dimensional reconstruction of human corneal lamellae. Femtosecond-laser photodisruption at 60 kHz results in rougher surfaces compared with mechanical dissection. The motor-driven linear microkeratome allows single-pass dissection of ultrathin endothelial lamellae with a standard deviation
- surface metrology
- ultrathin grafts
- endothelial keratoplasty
- femtosecond laser
- mechanical microkeratome
- ATOMIC-FORCE MICROSCOPY
- CORNEAL STROMA