Superior in vivo compatibility of hydrophilic polymer coated prosthetic vascular grafts

Aart C. Strang*, Menno L. W. Knetsch, Mirza M. Idu, Radjesh J. Bisoendial, Gertjan Kramer, Dave Speijer, Leo. H. Koole, Erik S. G. Stroes, Joris. I. Rotmans

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Web of Science)

Abstract

Purpose: Protein adsorption, cell adhesion and graft patency was compared in hydrophilic versus hydrophobic polymer-coated prosthetic vascular grafts. We hypothesize that in vivo compatibility of hydrophilic polymer-coated prosthetic vascular grafts is superior to in vivo compatibility of hydrophobic grafts. Methods: A pairwise side-to-side common carotid artery interposition graft was placed eight female landrace goats ( mean weight 55 kg). Protein adsorption was assessed using Western Blot in two hydrophilic and two hydrophobic grafts harvested after three days. Graft patency was monitored for 28 days in six goats with continuous wave Doppler ultrasonography. Adherence of endothelial cells, leukocytes and platelets was determined with ELISA and compared between the two graft types after 28 days. Results: After three days, more ApoA-I, albumin and VEGF and less fibrin adsorbed to hydrophilic grafts. After 28 days, compared to hydrophobic grafts, higher numbers of endothelial cells were present on hydrophilic grafts (P=0.016), and less thrombocytes and leukocytes (P=0.012 and 0.024, respectively). Two out of eight hydrophobic grafts lost patency, while none of the hydrophilic grafts failed (P=0.157). Conclusions: Hydrophilic polymer-coated vascular grafts have superior in vivo compatibility when compared to hydrophobic grafts as characterized by reduced platelet and leukocyte adherence as well as higher endothelialization.
Original languageEnglish
Pages (from-to)95-101
JournalJournal of vascular access
Volume15
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • Endothelialization
  • Graft
  • Hydrophilic
  • Thrombosis
  • Vascular graft

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