Structure Dependent-Immunomodulation by Sugar Beet Arabinans via a SYK Tyrosine Kinase-Dependent Signaling Pathway

Marjolein Meijerink, Christiane Rosch, Nico Taverne, Koen Venema, Harry Gruppen, Henk A. Schols, Jerry M. Wells*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Web of Science)

Abstract

There is much interest in the immunomodulatory properties of dietary fibers but their activity may be influenced by contamination with microbial-associated molecular patterns (MAMPs) such as lipopolysaccharide (LPS) and lipoteichoic acids, which are difficult to remove completely from biological samples. Bone marrow-derived dendritic cells (BMDCs) from TLR2x4 double-KO mice were shown to be a reliable approach to analyse the immunomodulatory properties of a diverse range of dietary fibers, by avoiding immune cell activation due to contaminating MAMPs. Several of the 44 tested dietary fiber preparations induced cytokine responses in BMDCs from TLR2x4 double-KO mice. The particulate fractions of linear arabinan (LA) and branched arabinan (BA) from sugar beet pectin were shown to be strongly immune stimulatory with LA being more immune stimulatory than BA. Enzymatic debranching of BA increased its immune stimulatory activity, possibly due to increased particle formation by the alignment of debranched linear arabinan. Mechanistic studies showed that the immunostimulatory activity of LA and BA was independent of the Dectin-1 recognition but Syk kinase-dependent.
Original languageEnglish
Article number1972
Number of pages15
JournalFrontiers in Immunology
Volume9
DOIs
Publication statusPublished - 12 Oct 2018

Keywords

  • immunomodulation
  • dietary fiber
  • arabinans
  • pectin
  • structure-function relationship
  • C-type lectin receptors
  • CHAIN FATTY-ACIDS
  • VITRO MACROPHAGE ACTIVATION
  • BACTERIAL LIPOPROTEINS
  • ANTIFUNGAL IMMUNITY
  • DENDRITIC CELLS
  • POLYSACCHARIDES
  • MICROBIOTA
  • DECTIN-1
  • LIPOPOLYSACCHARIDES
  • VARIABILITY

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