Strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock

M.D.P. Luyer, W.A. Buurman, M. Hadfoune, G. Speelmans, J. Knol, J.A. Jacobs, C.H. Dejong, A.J. Vriesema, J.W. Greve

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Abstract

Probiotic therapy modulates the composition of the intestinal flora and inhibits the inflammatory response. These properties may be of benefit in the preservation of gut barrier integrity after injury or stress. In this study, we examined the effect of two Lactobacillus strains selected for their pathogen exclusion properties on intestinal barrier integrity following hemorrhagic shock. Additionally, the responsiveness of the macrophage cell line RAW 264.7 to combined exposure to Lactobacillus DNA or oligodeoxynucleotides containing CpG motifs (CpG-ODN) and endotoxin was assessed by measuring tumor necrosis factor alpha (TNF-alpha) release. Rats were administered lactobacilli (5 x 10(9) CFU) or vehicle for 7 days and were subjected subsequently to hemorrhagic shock by withdrawal of 2.1 ml blood/100 g tissue. Levels of plasma endotoxin, bacterial translocation to distant organs, and filamentous actin (F-actin) in the ileum were determined 24 h later. Rats treated with Lactobacillus rhamnosus showed reduced levels of plasma endotoxin (8 +/- 2 pg/ml versus 24 +/- 4 pg/ml; P = 0.01), bacterial translocation (2 CFU/gram versus 369 CFU/gram; P < 0.01), and disruption of F-actin distribution following hemorrhagic shock compared with nontreated control rats. In contrast, pretreatment with Lactobacillus fermentum had no substantial effect on gut barrier integrity. Interestingly, DNA preparations from both lactobacilli reduced endotoxin-induced TNF-alpha release dose dependently, whereas CpG-ODN increased TNF-alpha release. In conclusion, the pathogen exclusion properties of both Lactobacillus strains and the reduction of endotoxin-induced inflammation by their DNA in vitro are not prerequisites for a beneficial effect of probiotic therapy on gut barrier function following hemorrhagic shock. Although pretreatment with Lactobacillus spp. may be useful to preserve gut barrier integrity following severe hypotension, a thorough assessment of specific strains seems to be essential.
Original languageEnglish
Pages (from-to)3686-3692
JournalInfection and Immunity
Volume73
Issue number6
DOIs
Publication statusPublished - 1 Jan 2005

Cite this

Luyer, M.D.P. ; Buurman, W.A. ; Hadfoune, M. ; Speelmans, G. ; Knol, J. ; Jacobs, J.A. ; Dejong, C.H. ; Vriesema, A.J. ; Greve, J.W. / Strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock. In: Infection and Immunity. 2005 ; Vol. 73, No. 6. pp. 3686-3692.
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Strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock. / Luyer, M.D.P.; Buurman, W.A.; Hadfoune, M.; Speelmans, G.; Knol, J.; Jacobs, J.A.; Dejong, C.H.; Vriesema, A.J.; Greve, J.W.

In: Infection and Immunity, Vol. 73, No. 6, 01.01.2005, p. 3686-3692.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock

AU - Luyer, M.D.P.

AU - Buurman, W.A.

AU - Hadfoune, M.

AU - Speelmans, G.

AU - Knol, J.

AU - Jacobs, J.A.

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AU - Greve, J.W.

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N2 - Probiotic therapy modulates the composition of the intestinal flora and inhibits the inflammatory response. These properties may be of benefit in the preservation of gut barrier integrity after injury or stress. In this study, we examined the effect of two Lactobacillus strains selected for their pathogen exclusion properties on intestinal barrier integrity following hemorrhagic shock. Additionally, the responsiveness of the macrophage cell line RAW 264.7 to combined exposure to Lactobacillus DNA or oligodeoxynucleotides containing CpG motifs (CpG-ODN) and endotoxin was assessed by measuring tumor necrosis factor alpha (TNF-alpha) release. Rats were administered lactobacilli (5 x 10(9) CFU) or vehicle for 7 days and were subjected subsequently to hemorrhagic shock by withdrawal of 2.1 ml blood/100 g tissue. Levels of plasma endotoxin, bacterial translocation to distant organs, and filamentous actin (F-actin) in the ileum were determined 24 h later. Rats treated with Lactobacillus rhamnosus showed reduced levels of plasma endotoxin (8 +/- 2 pg/ml versus 24 +/- 4 pg/ml; P = 0.01), bacterial translocation (2 CFU/gram versus 369 CFU/gram; P < 0.01), and disruption of F-actin distribution following hemorrhagic shock compared with nontreated control rats. In contrast, pretreatment with Lactobacillus fermentum had no substantial effect on gut barrier integrity. Interestingly, DNA preparations from both lactobacilli reduced endotoxin-induced TNF-alpha release dose dependently, whereas CpG-ODN increased TNF-alpha release. In conclusion, the pathogen exclusion properties of both Lactobacillus strains and the reduction of endotoxin-induced inflammation by their DNA in vitro are not prerequisites for a beneficial effect of probiotic therapy on gut barrier function following hemorrhagic shock. Although pretreatment with Lactobacillus spp. may be useful to preserve gut barrier integrity following severe hypotension, a thorough assessment of specific strains seems to be essential.

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DO - 10.1128/IAI.73.6.3686-3692.2005

M3 - Article

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