Starvation compromises paneth cells

C.M.I. Hodin, K. Lenaerts, J. Grootjans, J. de Haan, M.h. Hadfoune, F.K. Verheyen, H. Kiyama, E. Heineman, W.A. Buurman

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

Lack of enteral feeding, with or without parenteral nutritional support, is associated with increased intestinal permeability and translocation of bacteria. Such translocation is thought to be important in the high morbidity and mortality rates of patients who receive nothing by mouth. Recently, Paneth cells, important constituents of innate intestinal immunity, were found to be crucial in host protection against invasion of both commensal and pathogenic bacteria. This study investigates the influence of food deprivation on Paneth cell function in a mouse starvation model. Quantitative PCR showed significant decreases in mRNA expression of typical Paneth cell antimicrobials, lysozyme, cryptdin, and RegIIIgamma, in ileal tissue after 48 hours of food deprivation. Protein expression levels of lysozyme and RegIIIgamma precursor were also significantly diminished, as shown by Western blot analysis and IHC. Late degenerative autophagolysosomes and aberrant Paneth cell granules in starved mice were evident by electron microscopy, Western blot analysis, and quantitative PCR. Furthermore, increased bacterial translocation to mesenteric lymph nodes coincided with Paneth cell abnormalities. The current study demonstrates the occurrence of Paneth cell abnormalities during enteral starvation. Such changes may contribute to loss of epithelial barrier function, causing the apparent bacterial translocation in enteral starvation.
Original languageEnglish
Pages (from-to)2885-2893
JournalAmerican Journal of Pathology
Volume179
Issue number6
DOIs
Publication statusPublished - 1 Jan 2011

Cite this

Hodin, C. M. I., Lenaerts, K., Grootjans, J., de Haan, J., Hadfoune, M. H., Verheyen, F. K., ... Buurman, W. A. (2011). Starvation compromises paneth cells. American Journal of Pathology, 179(6), 2885-2893. https://doi.org/10.1016/j.ajpath.2011.08.030
Hodin, C.M.I. ; Lenaerts, K. ; Grootjans, J. ; de Haan, J. ; Hadfoune, M.h. ; Verheyen, F.K. ; Kiyama, H. ; Heineman, E. ; Buurman, W.A. / Starvation compromises paneth cells. In: American Journal of Pathology. 2011 ; Vol. 179, No. 6. pp. 2885-2893.
@article{d13be4f907274dbab25f42ec4daa390e,
title = "Starvation compromises paneth cells",
abstract = "Lack of enteral feeding, with or without parenteral nutritional support, is associated with increased intestinal permeability and translocation of bacteria. Such translocation is thought to be important in the high morbidity and mortality rates of patients who receive nothing by mouth. Recently, Paneth cells, important constituents of innate intestinal immunity, were found to be crucial in host protection against invasion of both commensal and pathogenic bacteria. This study investigates the influence of food deprivation on Paneth cell function in a mouse starvation model. Quantitative PCR showed significant decreases in mRNA expression of typical Paneth cell antimicrobials, lysozyme, cryptdin, and RegIIIgamma, in ileal tissue after 48 hours of food deprivation. Protein expression levels of lysozyme and RegIIIgamma precursor were also significantly diminished, as shown by Western blot analysis and IHC. Late degenerative autophagolysosomes and aberrant Paneth cell granules in starved mice were evident by electron microscopy, Western blot analysis, and quantitative PCR. Furthermore, increased bacterial translocation to mesenteric lymph nodes coincided with Paneth cell abnormalities. The current study demonstrates the occurrence of Paneth cell abnormalities during enteral starvation. Such changes may contribute to loss of epithelial barrier function, causing the apparent bacterial translocation in enteral starvation.",
author = "C.M.I. Hodin and K. Lenaerts and J. Grootjans and {de Haan}, J. and M.h. Hadfoune and F.K. Verheyen and H. Kiyama and E. Heineman and W.A. Buurman",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/j.ajpath.2011.08.030",
language = "English",
volume = "179",
pages = "2885--2893",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Science",
number = "6",

}

Hodin, CMI, Lenaerts, K, Grootjans, J, de Haan, J, Hadfoune, MH, Verheyen, FK, Kiyama, H, Heineman, E & Buurman, WA 2011, 'Starvation compromises paneth cells', American Journal of Pathology, vol. 179, no. 6, pp. 2885-2893. https://doi.org/10.1016/j.ajpath.2011.08.030

Starvation compromises paneth cells. / Hodin, C.M.I.; Lenaerts, K.; Grootjans, J.; de Haan, J.; Hadfoune, M.h.; Verheyen, F.K.; Kiyama, H.; Heineman, E.; Buurman, W.A.

In: American Journal of Pathology, Vol. 179, No. 6, 01.01.2011, p. 2885-2893.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Starvation compromises paneth cells

AU - Hodin, C.M.I.

AU - Lenaerts, K.

AU - Grootjans, J.

AU - de Haan, J.

AU - Hadfoune, M.h.

AU - Verheyen, F.K.

AU - Kiyama, H.

AU - Heineman, E.

AU - Buurman, W.A.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Lack of enteral feeding, with or without parenteral nutritional support, is associated with increased intestinal permeability and translocation of bacteria. Such translocation is thought to be important in the high morbidity and mortality rates of patients who receive nothing by mouth. Recently, Paneth cells, important constituents of innate intestinal immunity, were found to be crucial in host protection against invasion of both commensal and pathogenic bacteria. This study investigates the influence of food deprivation on Paneth cell function in a mouse starvation model. Quantitative PCR showed significant decreases in mRNA expression of typical Paneth cell antimicrobials, lysozyme, cryptdin, and RegIIIgamma, in ileal tissue after 48 hours of food deprivation. Protein expression levels of lysozyme and RegIIIgamma precursor were also significantly diminished, as shown by Western blot analysis and IHC. Late degenerative autophagolysosomes and aberrant Paneth cell granules in starved mice were evident by electron microscopy, Western blot analysis, and quantitative PCR. Furthermore, increased bacterial translocation to mesenteric lymph nodes coincided with Paneth cell abnormalities. The current study demonstrates the occurrence of Paneth cell abnormalities during enteral starvation. Such changes may contribute to loss of epithelial barrier function, causing the apparent bacterial translocation in enteral starvation.

AB - Lack of enteral feeding, with or without parenteral nutritional support, is associated with increased intestinal permeability and translocation of bacteria. Such translocation is thought to be important in the high morbidity and mortality rates of patients who receive nothing by mouth. Recently, Paneth cells, important constituents of innate intestinal immunity, were found to be crucial in host protection against invasion of both commensal and pathogenic bacteria. This study investigates the influence of food deprivation on Paneth cell function in a mouse starvation model. Quantitative PCR showed significant decreases in mRNA expression of typical Paneth cell antimicrobials, lysozyme, cryptdin, and RegIIIgamma, in ileal tissue after 48 hours of food deprivation. Protein expression levels of lysozyme and RegIIIgamma precursor were also significantly diminished, as shown by Western blot analysis and IHC. Late degenerative autophagolysosomes and aberrant Paneth cell granules in starved mice were evident by electron microscopy, Western blot analysis, and quantitative PCR. Furthermore, increased bacterial translocation to mesenteric lymph nodes coincided with Paneth cell abnormalities. The current study demonstrates the occurrence of Paneth cell abnormalities during enteral starvation. Such changes may contribute to loss of epithelial barrier function, causing the apparent bacterial translocation in enteral starvation.

U2 - 10.1016/j.ajpath.2011.08.030

DO - 10.1016/j.ajpath.2011.08.030

M3 - Article

VL - 179

SP - 2885

EP - 2893

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 6

ER -

Hodin CMI, Lenaerts K, Grootjans J, de Haan J, Hadfoune MH, Verheyen FK et al. Starvation compromises paneth cells. American Journal of Pathology. 2011 Jan 1;179(6):2885-2893. https://doi.org/10.1016/j.ajpath.2011.08.030