Standardized, modular parallelization platform for microfluidic large-scale integration cell culturing chips

Anke R. Vollertsen; Stefan Dekker; Britt A.M. Wesselink; Rob Haverkate; Johan G. Bomer; Hoon Suk Rho; Robert Jan Boom; Maciej Skolimowski; Marko Blom; Andries D. Van Der Meer; Robert Passier; Albert Van Den Berg; Mathieu Odijk

Standardized, modular parallelization platform for microfluidic large-scale integration cell culturing chips

Anke R. Vollertsen^*, Stefan Dekker, Britt A.M. Wesselink, Rob Haverkate, Johan G. Bomer, Hoon Suk Rho, Robert Jan Boom, Maciej Skolimowski, Marko Blom, Andries D. Van Der Meer, Robert Passier, Albert Van Den Berg, Mathieu Odijk

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

Abstract

Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher	Chemical and Biological Microsystems Society
Pages	2199-2201
Number of pages	3
Volume	4
ISBN (Electronic)	9781510897571
Publication status	Published - 1 Jan 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences - Kaohsiung, Taiwan Duration: 11 Nov 2018 → 15 Nov 2018 Conference number: 22

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	11/11/18 → 15/11/18

Keywords

Cell culture
Microfluidic large-scale integration
Parallelized
Standardization

Cite this

Vollertsen, A. R., Dekker, S., Wesselink, B. A. M., Haverkate, R., Bomer, J. G., Rho, H. S., Boom, R. J., Skolimowski, M., Blom, M., Van Der Meer, A. D., Passier, R., Van Den Berg, A., & Odijk, M. (2018). Standardized, modular parallelization platform for microfluidic large-scale integration cell culturing chips. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (Vol. 4, pp. 2199-2201). Chemical and Biological Microsystems Society.

@inproceedings{860308487bb34dc0ad422b4efeb2f76d,

title = "Standardized, modular parallelization platform for microfluidic large-scale integration cell culturing chips",

abstract = "Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).",

keywords = "Cell culture, Microfluidic large-scale integration, Parallelized, Standardization",

author = "Vollertsen, {Anke R.} and Stefan Dekker and Wesselink, {Britt A.M.} and Rob Haverkate and Bomer, {Johan G.} and Rho, {Hoon Suk} and Boom, {Robert Jan} and Maciej Skolimowski and Marko Blom and {Van Der Meer}, {Andries D.} and Robert Passier and {Van Den Berg}, Albert and Mathieu Odijk",

note = "Funding Information: This work was supported by the VESCEL ERC Advanced Grant to A. van den Berg (Grant no. 669768) and the MFManufacturing ESCEL Joint Undertaking (Grant no. 621275-2). Publisher Copyright: Copyright {\textcopyright} (2018) by Chemical and Biological Microsystems Society. All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

year = "2018",

month = jan,

day = "1",

language = "English",

volume = "4",

pages = "2199--2201",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Chemical and Biological Microsystems Society",

address = "United States",

}

Vollertsen, AR, Dekker, S, Wesselink, BAM, Haverkate, R, Bomer, JG, Rho, HS, Boom, RJ, Skolimowski, M, Blom, M, Van Der Meer, AD, Passier, R, Van Den Berg, A & Odijk, M 2018, Standardized, modular parallelization platform for microfluidic large-scale integration cell culturing chips. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. vol. 4, Chemical and Biological Microsystems Society, pp. 2199-2201, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, Kaohsiung, Taiwan, 11/11/18.

Standardized, modular parallelization platform for microfluidic large-scale integration cell culturing chips. / Vollertsen, Anke R.; Dekker, Stefan; Wesselink, Britt A.M. et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Vol. 4 Chemical and Biological Microsystems Society, 2018. p. 2199-2201.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

TY - GEN

T1 - Standardized, modular parallelization platform for microfluidic large-scale integration cell culturing chips

AU - Vollertsen, Anke R.

AU - Dekker, Stefan

AU - Wesselink, Britt A.M.

AU - Haverkate, Rob

AU - Bomer, Johan G.

AU - Rho, Hoon Suk

AU - Boom, Robert Jan

AU - Skolimowski, Maciej

AU - Blom, Marko

AU - Van Der Meer, Andries D.

AU - Passier, Robert

AU - Van Den Berg, Albert

AU - Odijk, Mathieu

N1 - Conference code: 22

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).

AB - Standardized high-throughput devices for microfluidic cell cultures are necessary to translate discoveries made in academia to applications in pharmaceutical industry. Here we present a platform with integrated pneumatic valves for standardized parallelization of multichamber chips (SPARC). In total, 192 chambers divided over three microfluidic building blocks (MFBBs) can be filled and purged with spatial and temporal independence. The dimensions of both the MFBB and the platform are standardized and thus compatible with common lab equipment. We characterize the valves at different pumping and gate pressures and show that the MFBBs are suitable for culturing human umbilical vein endothelial cells (HUVECs).

KW - Cell culture

KW - Microfluidic large-scale integration

KW - Parallelized

KW - Standardization

UR - http://www.scopus.com/inward/record.url?scp=85079818714&partnerID=8YFLogxK

M3 - Conference article in proceeding

VL - 4

SP - 2199

EP - 2201

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 11 November 2018 through 15 November 2018

ER -

Standardized, modular parallelization platform for microfluidic large-scale integration cell culturing chips

Abstract

Conference

Keywords

Other files and links

Fingerprint

Cite this