Spectrophotometric method for the assay of human blood coagulation factor VIII

G van Dieijen, M.P. van Dieijen-Visser, J. Franssen, H.C. Hemker

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    Abstract

    A spectrophotometric method for the assay of human blood coagulation factor VIII in plasma is presented. The chromogenic assay for factor VIIL·C in plasma is performed in 3 steps: (1) activation of factor VIII by thrombin; (2) activation of factor X in a mixture of factor X, factor IXa, phospholipids/Ca2+ and plasma containing activated factor VIII, and (3) determination of the rate of factor Xa formation with the chromogenic substrate S2337. Within-assay variation was between 5 and 6.9% for factor VIIL·C activities between 20 and 150%. Clotting and chromogenic factor VIIL·C activities were compared in plasma of 50 normal healthy donors (coefficient of correlation r = 0.83).
    Original languageEnglish
    Pages (from-to)14-24
    Number of pages11
    JournalHaemostasis
    Volume17
    Issue number1-2
    DOIs
    Publication statusPublished - 1987

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