Spatially resolved proteomics in osteoarthritis: State of the art and new perspectives

M. R. Eveque-Mourroux; B. Rocha; F. P. Y. Barre; R. M. A. Heeren; B. Cillero-Pastor

doi:10.1016/j.jprot.2020.103637

Spatially resolved proteomics in osteoarthritis: State of the art and new perspectives

M. R. Eveque-Mourroux, B. Rocha, F. P. Y. Barre, R. M. A. Heeren, B. Cillero-Pastor^*

^*Corresponding author for this work

Research output: Contribution to journal › (Systematic) Review article › peer-review

Abstract

Osteoarthritis (OA) is one of the most common diseases worldwide caused by chronic degeneration of the joints. Its high prevalence and the involvement of several tissues define OA as a highly heterogeneous disease. New biological markers to evaluate the progression of the pathology and improve its prognosis are needed. Among all the different-omic strategies applied to OA, solution phase bottom-up proteomics has made an extensive contribution to the field of biomarker research. However, new technologies for protein analysis should be considered for a better understanding of the disease. This review focuses on complementary proteomic methodologies and new technologies for translational research of OA and other rheumatic pathologies, especially mass spectrometry imaging and protein imaging methods not applied by the OA community yet.

Original language	English
Article number	103637
Number of pages	7
Journal	Journal of Proteomics
Volume	215
DOIs	https://doi.org/10.1016/j.jprot.2020.103637
Publication status	Published - 20 Mar 2020

Keywords

Osteoarthritis
Proteomics
Molecular imaging
Spatially resolved
LASER CAPTURE MICRODISSECTION
IMAGING MASS-SPECTROMETRY
SYNOVIAL-FLUID
OSTEOCHONDRAL JUNCTION
EXTRACELLULAR-MATRIX
HUMAN INTERACTOME
SUBCHONDRAL BONE
N-GLYCANS
TOP-DOWN
TISSUE

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Cite this

@article{c18775a46ef74f3f81292967b9a12122,

title = "Spatially resolved proteomics in osteoarthritis: State of the art and new perspectives",

abstract = "Osteoarthritis (OA) is one of the most common diseases worldwide caused by chronic degeneration of the joints. Its high prevalence and the involvement of several tissues define OA as a highly heterogeneous disease. New biological markers to evaluate the progression of the pathology and improve its prognosis are needed. Among all the different-omic strategies applied to OA, solution phase bottom-up proteomics has made an extensive contribution to the field of biomarker research. However, new technologies for protein analysis should be considered for a better understanding of the disease. This review focuses on complementary proteomic methodologies and new technologies for translational research of OA and other rheumatic pathologies, especially mass spectrometry imaging and protein imaging methods not applied by the OA community yet.",

keywords = "Osteoarthritis, Proteomics, Molecular imaging, Spatially resolved, LASER CAPTURE MICRODISSECTION, IMAGING MASS-SPECTROMETRY, SYNOVIAL-FLUID, OSTEOCHONDRAL JUNCTION, EXTRACELLULAR-MATRIX, HUMAN INTERACTOME, SUBCHONDRAL BONE, N-GLYCANS, TOP-DOWN, TISSUE",

author = "Eveque-Mourroux, {M. R.} and B. Rocha and Barre, {F. P. Y.} and Heeren, {R. M. A.} and B. Cillero-Pastor",

note = "Funding Information: This work has been performed as part of the M4I research program that was financially supported by the Dutch Province of Limburg through the “LINK” program and the MUMC institutional grant for clinical collaborative research. B.R. was supported by Xunta de Galicia ( IN606B-2016/004 ). We acknowledge Dr. Pieter Emans for his support on sample collection. Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = mar,

day = "20",

doi = "10.1016/j.jprot.2020.103637",

language = "English",

volume = "215",

journal = "Journal of Proteomics",

issn = "1874-3919",

publisher = "Elsevier Science",

}

TY - JOUR

T1 - Spatially resolved proteomics in osteoarthritis

T2 - State of the art and new perspectives

AU - Eveque-Mourroux, M. R.

AU - Rocha, B.

AU - Barre, F. P. Y.

AU - Heeren, R. M. A.

AU - Cillero-Pastor, B.

N1 - Funding Information: This work has been performed as part of the M4I research program that was financially supported by the Dutch Province of Limburg through the “LINK” program and the MUMC institutional grant for clinical collaborative research. B.R. was supported by Xunta de Galicia ( IN606B-2016/004 ). We acknowledge Dr. Pieter Emans for his support on sample collection. Publisher Copyright: © 2020 The Authors

PY - 2020/3/20

Y1 - 2020/3/20

N2 - Osteoarthritis (OA) is one of the most common diseases worldwide caused by chronic degeneration of the joints. Its high prevalence and the involvement of several tissues define OA as a highly heterogeneous disease. New biological markers to evaluate the progression of the pathology and improve its prognosis are needed. Among all the different-omic strategies applied to OA, solution phase bottom-up proteomics has made an extensive contribution to the field of biomarker research. However, new technologies for protein analysis should be considered for a better understanding of the disease. This review focuses on complementary proteomic methodologies and new technologies for translational research of OA and other rheumatic pathologies, especially mass spectrometry imaging and protein imaging methods not applied by the OA community yet.

AB - Osteoarthritis (OA) is one of the most common diseases worldwide caused by chronic degeneration of the joints. Its high prevalence and the involvement of several tissues define OA as a highly heterogeneous disease. New biological markers to evaluate the progression of the pathology and improve its prognosis are needed. Among all the different-omic strategies applied to OA, solution phase bottom-up proteomics has made an extensive contribution to the field of biomarker research. However, new technologies for protein analysis should be considered for a better understanding of the disease. This review focuses on complementary proteomic methodologies and new technologies for translational research of OA and other rheumatic pathologies, especially mass spectrometry imaging and protein imaging methods not applied by the OA community yet.

KW - Osteoarthritis

KW - Proteomics

KW - Molecular imaging

KW - Spatially resolved

KW - LASER CAPTURE MICRODISSECTION

KW - IMAGING MASS-SPECTROMETRY

KW - SYNOVIAL-FLUID

KW - OSTEOCHONDRAL JUNCTION

KW - EXTRACELLULAR-MATRIX

KW - HUMAN INTERACTOME

KW - SUBCHONDRAL BONE

KW - N-GLYCANS

KW - TOP-DOWN

KW - TISSUE

U2 - 10.1016/j.jprot.2020.103637

DO - 10.1016/j.jprot.2020.103637

M3 - (Systematic) Review article

C2 - 31926309

SN - 1874-3919

VL - 215

JO - Journal of Proteomics

JF - Journal of Proteomics

M1 - 103637

ER -