@article{1e48f5a3ac3a45438e69958489a554b2,
title = "Spatial lipidomics reveals biased phospholipid remodeling in acute Pseudomonas lung infection",
abstract = "Pseudomonas aeruginosa (Pa) is a pathogen causing chronic pulmonary infections in patients with cystic fibrosis (CF). Manipulation of lipids is an important feature of Pa infection and on a tissue-level scale is poorly understood. Using a mouse model of acute Pa pulmonary infection, we explored the whole-lung phospholipid response using mass spectrometry imaging (MSI) and spatial lipidomics. Using a histology-driven analysis, we isolated airways and parenchyma from both mock- and Pa-infected lungs and used systems biology tools to identify enriched metabolic pathways from the differential phospholipid identities. Infection was associated with a set of 26 ions, with 11 unique to parenchyma and 6 unique to airways. Acyl remodeling was differentially enriched in infected parenchyma as the predominant biological function. These functions correlated with markers of polymorphonuclear (PMN) cell influx, a defining feature of the lung response to Pa infection, implicating enzymes active in phospholipid remodeling.",
keywords = "Biological sciences, Lipidomics, Microbiology",
author = "Scott, {Alison J.} and Ellis, {Shane R.} and Hofstaedter, {Casey E.} and Heeren, {Ron M.A.} and Ernst, {Robert K.}",
note = "Funding Information: This work was supported by CFF ERNST18I0 to R.K.E. and NIH/NIAID AI177823 to A.J.S. and R.K.E. R.M.A.H. and S.R.E. acknowledge financial support from the LINK program of the Dutch province of Limburg. S.R.E. acknowledges funding from the Australian Research Council Future Fellowship Scheme (grant number FT190100082) and the Netherlands Organization for Scientific Research VIDI scheme (grant number 198.011). Graphical abstract created in BioRender. Conceptualization, A.J.S. S.R.E. R.M.A.H. R.K.E.; Formal Analysis, A.J.S. S.R.E.; Investigation, A.J.S. S.R.E.; Resources, A.J.S. S.R.E. R.M.A.H. R.K.E.; Writing – Original Draft, A.J.S.; Writing – Review & Editing, A.J.S. S.R.E. C.E.H. R.M.A.H. R.K.E.; Visualization, A.J.S. S.R.E.; Project Administration, A.J.S.; Funding Acquisition, R.M.A.H. R.K.E. The authors declare no conflict of interest in this work. One or more of the authors of this paper self-identifies as a gender minority in their field of research. One or more of the authors of this paper self-identifies as a member of the LGBTQIA+ community. We support inclusive, diverse, and equitable conduct of research. Funding Information: This work was supported by CFF ERNST18I0 to R.K.E. and NIH /NIAID AI177823 to A.J.S. and R.K.E. R.M.A.H. and S.R.E. acknowledge financial support from the LINK program of the Dutch province of Limburg . S.R.E. acknowledges funding from the Australian Research Council Future Fellowship Scheme (grant number FT190100082 ) and the Netherlands Organization for Scientific Research VIDI scheme (grant number 198.011 ). Graphical abstract created in BioRender. Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = sep,
day = "15",
doi = "10.1016/j.isci.2023.107700",
language = "English",
volume = "26",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "9",
}