Sound-based assembly of three-dimensional cellularized and acellularized constructs

Riccardo Tognato, Romedi Parolini, Shahrbanoo Jahangir, Junxuan Ma, Sammy Florczak, R. Geoff Richards, Riccardo Levato, Mauro Alini, Tiziano Serra*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Herein we show an accessible technique based on Faraday waves that assist the rapid assembly of osteoinductive ß-Tricalcium phosphate (ß-TCP) particles as well as human osteoblast pre-assembled in spheroids. The hydrodynamic forces originating at ‘seabed’ of the assembly chamber can be used to tightly aggregate inorganic and biological entities at packing densities that resemble those of native tissues. Additionally, following a layer-by-layer assembly procedure, centimeter scaled osteoinductive three-dimensional and cellularized constructs have been fabricated. We showed that the intimate connection between biological building blocks is essential in engineering living system able of localized mineral deposition. Our results demonstrate, for the first time, the possibility to obtain three-dimensional cellularized and acellularized anisotropic constructs using Faraday waves.
Original languageEnglish
Article number100775
Number of pages10
JournalMaterials today. Bio
Volume22
DOIs
Publication statusPublished - 1 Oct 2023

Keywords

  • Acoustic
  • Biofabrication
  • Sound-based assembly

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