Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering

Alicia Fernández-Colino, Frederic Wolf, Stephan Rütten, Thomas Schmitz-Rode, Jose Carlos Rodríguez-Cabello, Stefan Jockenhoevel*, Petra Mela*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Web of Science)

Abstract

Vascular disease is a leading cause of death worldwide, but surgical options are restricted by the limited availability of autologous vessels, and the suboptimal performance of prosthetic vascular grafts. This is especially evident for coronary artery by-pass grafts, whose small caliber is associated with a high occlusion propensity. Despite the potential of tissue-engineered grafts, compliance mismatch, dilatation, thrombus formation, and the lack of functional elastin are still major limitations leading to graft failure. This calls for advanced materials and fabrication schemes to achieve improved control on the grafts' properties and performance. Here, bioinspired materials and technical textile components are combined to create biohybrid cell-free implants for endogenous tissue regeneration. Clickable elastin-like recombinamers are processed to form an open macroporous 3D architecture to favor cell ingrowth, while being endowed with the non-thrombogenicity and the elastic behavior of the native elastin. The textile components (i.e., warp-knitted and electrospun meshes) are designed to confer suture retention, long-term structural stability, burst strength, and compliance. Notably, by controlling the electrospun layer's thickness, the compliance can be modulated over a wide range of values encompassing those of native vessels. The grafts support cell ingrowth, extracellular matrix deposition and endothelium development in vitro. Overall, the fabrication strategy results in promising off-the-shelf hemocompatible vascular implants for in situ tissue engineering by addressing the known limitations of bioartificial vessel substitutes.

Original languageEnglish
Article number340
Number of pages13
JournalFrontiers in bioengineering and biotechnology
Volume7
DOIs
Publication statusPublished - 19 Nov 2019

Keywords

  • BLOOD-VESSELS
  • BYPASS GRAFTS
  • COMPLIANCE MISMATCH
  • ENDOTHELIUM
  • INTIMAL HYPERPLASIA
  • MECHANICAL-PROPERTIES
  • POLYPEPTIDES
  • SAPHENOUS-VEIN
  • SCAFFOLD
  • STIFFNESS
  • biohybrid scaffolds
  • elastin-like recombinamers
  • off-the-shelf implants
  • textile technical components
  • vascular grafts

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