Abstract
Two variants of Annexin A5 (Cys2-AnxA5 and Cys165-AnxA5) were labelled with Gallium-68 in order to evaluate their biological properties.Biodistribution and pharmacokinetics of the radiotracers were studied with ?PET in healthy mice and in a mouse model of hepatic apoptosis. ?PET imaging after IV injection of the tracers in combination with ?MRI was performed in Daudi tumor bearing mice before and after treatment with a combination of chemotherapy and radiotherapy.The biodistribution data indicated a fast urinary clearance with only minor hepatobilliary clearance, although a high retention in the kidneys was observed. Animals treated with anti-Fas showed a 3 to 8 times higher liver uptake as compared to healthy animals. Tumor uptake of (68)Ga-Cys2-AnxA5 and (68)Ga-Cys165-AnxA5 was low but significantly increased after therapy.Both (68)Ga-Cys2-AnxA5 and (68)Ga-Cys165-AnxA5 show a clear binding to apoptotic cells and are promising tracers for rapid evaluation of cancer therapy.
Original language | English |
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Pages (from-to) | 381-392 |
Journal | Nuclear Medicine and Biology |
Volume | 38 |
Issue number | 3 |
DOIs | |
Publication status | Published - Apr 2011 |
Keywords
- [Ga-68]
- Annexin A5
- Apoptosis
- Therapy evaluation
- PET