SIP-Based Thermal Detection Platform for the Direct Detection of Bacteria Obtained from a Contaminated Surface

Bart van Grinsven, Kasper Eersels, Sandra Erkens - Hulshof, Hanne Diliën, Kunya Nurekeyeva, Peter Cornelis, D Klein, F Crijns, Gabrielle Tuijthof, P. Wagner, Erik Steen Redeker, Thomas Cleij

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Surface detection of bacteria has been proven difficult and time-consuming. Different recovery techniques yield varying numbers of bacteria. Subsequently, bacterial culturing, used for identification of these bacteria, will take several hours. In this article, the potential of a newly developed thermal biomimetic sensor for the detection of bacteria on surfaces is described. Previously this thermal biomimetic sensor has proven to be able to detect and quantify different bacteria in various liquid media such as buffer and spiked urine samples. In this article, laboratory surfaces are contaminated with increasing concentrations of Escherichia coli. Bacteria are recovered from the surfaces using commercially available swab rinse kits (SRK). A calibration curve is created by coating chips with surface-imprinted polymers (SIPs), serving as synthetic bacteria receptors, and exposing them to increasing concentrations of E. coli. Next, concentrations of E. coli in the SRK buffer are measured and quantified. The results show that it is possible to detect E. coli recovered from surfaces. Although quantification has been proven difficult as the dynamic range of the sensor is relatively narrow and the bacterial load obtained by using SRK is low, the sensor is able to give an indication about the concentration present on the surface. The results in this article illustrate that the thermal biomimetic sensor is a fast, low-cost, and label-free device useful in surface detection of E. coli, and seems a promising tool for future on-site bacterial detection.

Original languageEnglish
Article number1700777
Pages (from-to)1-5
Number of pages5
JournalPhysica Status Solidi A-applications and Materials Science
Volume215
Issue number15
Early online date2018
DOIs
Publication statusPublished - 8 Aug 2018

Keywords

  • FILMS
  • HEAT-TRANSFER-METHOD
  • IMPRINTED POLYMERS
  • LABEL-FREE DETECTION
  • RECOGNITION
  • SYNTHETIC RECEPTORS
  • bacterial culture
  • bacterial detection
  • heat-transfer methods
  • swab rinse kits
  • thermal interface resistance

Cite this

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title = "SIP-Based Thermal Detection Platform for the Direct Detection of Bacteria Obtained from a Contaminated Surface",
abstract = "Surface detection of bacteria has been proven difficult and time-consuming. Different recovery techniques yield varying numbers of bacteria. Subsequently, bacterial culturing, used for identification of these bacteria, will take several hours. In this article, the potential of a newly developed thermal biomimetic sensor for the detection of bacteria on surfaces is described. Previously this thermal biomimetic sensor has proven to be able to detect and quantify different bacteria in various liquid media such as buffer and spiked urine samples. In this article, laboratory surfaces are contaminated with increasing concentrations of Escherichia coli. Bacteria are recovered from the surfaces using commercially available swab rinse kits (SRK). A calibration curve is created by coating chips with surface-imprinted polymers (SIPs), serving as synthetic bacteria receptors, and exposing them to increasing concentrations of E. coli. Next, concentrations of E. coli in the SRK buffer are measured and quantified. The results show that it is possible to detect E. coli recovered from surfaces. Although quantification has been proven difficult as the dynamic range of the sensor is relatively narrow and the bacterial load obtained by using SRK is low, the sensor is able to give an indication about the concentration present on the surface. The results in this article illustrate that the thermal biomimetic sensor is a fast, low-cost, and label-free device useful in surface detection of E. coli, and seems a promising tool for future on-site bacterial detection.",
keywords = "FILMS, HEAT-TRANSFER-METHOD, IMPRINTED POLYMERS, LABEL-FREE DETECTION, RECOGNITION, SYNTHETIC RECEPTORS, bacterial culture, bacterial detection, heat-transfer methods, swab rinse kits, thermal interface resistance",
author = "{van Grinsven}, Bart and Kasper Eersels and {Erkens - Hulshof}, Sandra and Hanne Dili{\"e}n and Kunya Nurekeyeva and Peter Cornelis and D Klein and F Crijns and Gabrielle Tuijthof and P. Wagner and {Steen Redeker}, Erik and Thomas Cleij",
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journal = "Physica Status Solidi A-applications and Materials Science",
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SIP-Based Thermal Detection Platform for the Direct Detection of Bacteria Obtained from a Contaminated Surface. / van Grinsven, Bart; Eersels, Kasper; Erkens - Hulshof, Sandra; Diliën, Hanne; Nurekeyeva, Kunya; Cornelis, Peter; Klein, D; Crijns, F; Tuijthof, Gabrielle; Wagner, P.; Steen Redeker, Erik; Cleij, Thomas.

In: Physica Status Solidi A-applications and Materials Science, Vol. 215, No. 15, 1700777, 08.08.2018, p. 1-5.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - SIP-Based Thermal Detection Platform for the Direct Detection of Bacteria Obtained from a Contaminated Surface

AU - van Grinsven, Bart

AU - Eersels, Kasper

AU - Erkens - Hulshof, Sandra

AU - Diliën, Hanne

AU - Nurekeyeva, Kunya

AU - Cornelis, Peter

AU - Klein, D

AU - Crijns, F

AU - Tuijthof, Gabrielle

AU - Wagner, P.

AU - Steen Redeker, Erik

AU - Cleij, Thomas

PY - 2018/8/8

Y1 - 2018/8/8

N2 - Surface detection of bacteria has been proven difficult and time-consuming. Different recovery techniques yield varying numbers of bacteria. Subsequently, bacterial culturing, used for identification of these bacteria, will take several hours. In this article, the potential of a newly developed thermal biomimetic sensor for the detection of bacteria on surfaces is described. Previously this thermal biomimetic sensor has proven to be able to detect and quantify different bacteria in various liquid media such as buffer and spiked urine samples. In this article, laboratory surfaces are contaminated with increasing concentrations of Escherichia coli. Bacteria are recovered from the surfaces using commercially available swab rinse kits (SRK). A calibration curve is created by coating chips with surface-imprinted polymers (SIPs), serving as synthetic bacteria receptors, and exposing them to increasing concentrations of E. coli. Next, concentrations of E. coli in the SRK buffer are measured and quantified. The results show that it is possible to detect E. coli recovered from surfaces. Although quantification has been proven difficult as the dynamic range of the sensor is relatively narrow and the bacterial load obtained by using SRK is low, the sensor is able to give an indication about the concentration present on the surface. The results in this article illustrate that the thermal biomimetic sensor is a fast, low-cost, and label-free device useful in surface detection of E. coli, and seems a promising tool for future on-site bacterial detection.

AB - Surface detection of bacteria has been proven difficult and time-consuming. Different recovery techniques yield varying numbers of bacteria. Subsequently, bacterial culturing, used for identification of these bacteria, will take several hours. In this article, the potential of a newly developed thermal biomimetic sensor for the detection of bacteria on surfaces is described. Previously this thermal biomimetic sensor has proven to be able to detect and quantify different bacteria in various liquid media such as buffer and spiked urine samples. In this article, laboratory surfaces are contaminated with increasing concentrations of Escherichia coli. Bacteria are recovered from the surfaces using commercially available swab rinse kits (SRK). A calibration curve is created by coating chips with surface-imprinted polymers (SIPs), serving as synthetic bacteria receptors, and exposing them to increasing concentrations of E. coli. Next, concentrations of E. coli in the SRK buffer are measured and quantified. The results show that it is possible to detect E. coli recovered from surfaces. Although quantification has been proven difficult as the dynamic range of the sensor is relatively narrow and the bacterial load obtained by using SRK is low, the sensor is able to give an indication about the concentration present on the surface. The results in this article illustrate that the thermal biomimetic sensor is a fast, low-cost, and label-free device useful in surface detection of E. coli, and seems a promising tool for future on-site bacterial detection.

KW - FILMS

KW - HEAT-TRANSFER-METHOD

KW - IMPRINTED POLYMERS

KW - LABEL-FREE DETECTION

KW - RECOGNITION

KW - SYNTHETIC RECEPTORS

KW - bacterial culture

KW - bacterial detection

KW - heat-transfer methods

KW - swab rinse kits

KW - thermal interface resistance

U2 - 10.1002/pssa.201700777

DO - 10.1002/pssa.201700777

M3 - Article

VL - 215

SP - 1

EP - 5

JO - Physica Status Solidi A-applications and Materials Science

JF - Physica Status Solidi A-applications and Materials Science

SN - 1862-6300

IS - 15

M1 - 1700777

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