Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release

P C Bessa; E R Balmayor; H S Azevedo; S Nürnberger; M Casal; M van Griensven; R L Reis; H Redl

doi:10.1002/term.245

Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release

P C Bessa^*, E R Balmayor, H S Azevedo, S Nürnberger, M Casal, M van Griensven, R L Reis, H Redl

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for the delivery of BMP-2, BMP-9 or BMP-14. BMP-containing fibroin microparticles were prepared by a mild methodology using dropwise addition of ethanol, exhibiting mean diameters of 2.7 +/- 0.3 microm. Encapsulation efficiencies varied between 67.9 +/- 6.1 % and 97.7 +/- 2.0 % depending on the type and the amount of BMP loaded. Release kinetics showed that BMP-2, BMP-9 and BMP-14 were released in two phases profile, with a burst release in the first two days followed by a slower release, for a period of 14 days. The release data were best explained by Korsmeyer's model and the Fickian model of drug diffusion. Silk fibroin microparticles can offer a promising approach for the sustained delivery of different BMPs in tissue engineering applications.

Original language	English
Pages (from-to)	349-355
Number of pages	7
Journal	Journal of Tissue Engineering and Regenerative Medicine
Volume	4
Issue number	5
DOIs	https://doi.org/10.1002/term.245
Publication status	Published - Jul 2010
Externally published	Yes

Keywords

Animals
Bone Morphogenetic Proteins/pharmacology
Drug Carriers/chemistry
Drug Delivery Systems/methods
Fibroins/chemistry
Humans
Immobilized Proteins/pharmacology
Kinetics
Microscopy, Electron, Scanning
Microspheres
Particle Size
Recombinant Proteins/pharmacology
Water/chemistry

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10.1002/term.245

Balmayor-2010-Silk_fibroin_microparticles_as_carriersFinal published version, 236 KBLicence: Taverne

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title = "Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release",

abstract = "Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for the delivery of BMP-2, BMP-9 or BMP-14. BMP-containing fibroin microparticles were prepared by a mild methodology using dropwise addition of ethanol, exhibiting mean diameters of 2.7 +/- 0.3 microm. Encapsulation efficiencies varied between 67.9 +/- 6.1 % and 97.7 +/- 2.0 % depending on the type and the amount of BMP loaded. Release kinetics showed that BMP-2, BMP-9 and BMP-14 were released in two phases profile, with a burst release in the first two days followed by a slower release, for a period of 14 days. The release data were best explained by Korsmeyer's model and the Fickian model of drug diffusion. Silk fibroin microparticles can offer a promising approach for the sustained delivery of different BMPs in tissue engineering applications.",

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author = "Bessa, {P C} and Balmayor, {E R} and Azevedo, {H S} and S N{\"u}rnberger and M Casal and {van Griensven}, M and Reis, {R L} and H Redl",

year = "2010",

month = jul,

doi = "10.1002/term.245",

language = "English",

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AU - Bessa, P C

AU - Balmayor, E R

AU - Azevedo, H S

AU - Nürnberger, S

AU - Casal, M

AU - van Griensven, M

AU - Reis, R L

AU - Redl, H

PY - 2010/7

Y1 - 2010/7

N2 - Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for the delivery of BMP-2, BMP-9 or BMP-14. BMP-containing fibroin microparticles were prepared by a mild methodology using dropwise addition of ethanol, exhibiting mean diameters of 2.7 +/- 0.3 microm. Encapsulation efficiencies varied between 67.9 +/- 6.1 % and 97.7 +/- 2.0 % depending on the type and the amount of BMP loaded. Release kinetics showed that BMP-2, BMP-9 and BMP-14 were released in two phases profile, with a burst release in the first two days followed by a slower release, for a period of 14 days. The release data were best explained by Korsmeyer's model and the Fickian model of drug diffusion. Silk fibroin microparticles can offer a promising approach for the sustained delivery of different BMPs in tissue engineering applications.

AB - Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for the delivery of BMP-2, BMP-9 or BMP-14. BMP-containing fibroin microparticles were prepared by a mild methodology using dropwise addition of ethanol, exhibiting mean diameters of 2.7 +/- 0.3 microm. Encapsulation efficiencies varied between 67.9 +/- 6.1 % and 97.7 +/- 2.0 % depending on the type and the amount of BMP loaded. Release kinetics showed that BMP-2, BMP-9 and BMP-14 were released in two phases profile, with a burst release in the first two days followed by a slower release, for a period of 14 days. The release data were best explained by Korsmeyer's model and the Fickian model of drug diffusion. Silk fibroin microparticles can offer a promising approach for the sustained delivery of different BMPs in tissue engineering applications.

KW - Animals

KW - Bone Morphogenetic Proteins/pharmacology

KW - Drug Carriers/chemistry

KW - Drug Delivery Systems/methods

KW - Fibroins/chemistry

KW - Humans

KW - Immobilized Proteins/pharmacology

KW - Kinetics

KW - Microscopy, Electron, Scanning

KW - Microspheres

KW - Particle Size

KW - Recombinant Proteins/pharmacology

KW - Water/chemistry

U2 - 10.1002/term.245

DO - 10.1002/term.245

M3 - Article

C2 - 20058243

SN - 1932-6254

VL - 4

SP - 349

EP - 355

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

IS - 5

ER -

Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release

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Keywords

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