Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate

A.R. Calore; V. Srinivas; S. Anand; A. Albillos-Sanchez; S.F.S.P. Looijmans; L.C.A. van Breemen; C. Mota; K. Bernaerts; J.A.W. Harings; L. Moroni

doi:10.1557/s43578-021-00403-2

Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate

A.R. Calore, V. Srinivas, S. Anand, A. Albillos-Sanchez, S.F.S.P. Looijmans, L.C.A. van Breemen, C. Mota, K. Bernaerts, J.A.W. Harings^*, L. Moroni^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Thermoplastic semi-crystalline polymers are excellent candidates for tissue engineering scaffolds thanks to facile processing and tunable properties, employed in melt-based additive manufacturing. Control of crystallization and ultimate crystallinity during processing affect properties like surface stiffness and roughness. These in turn influence cell attachment, proliferation and differentiation. Surface stiffness and roughness are intertwined via crystallinity, but never studied independently. The targeted stiffness range is besides difficult to realize for a single thermoplastic. Via correlation of thermal history, crystallization and ultimate crystallinity of vitamin E plasticized poly(lactide), surface stiffness and roughness are decoupled, disclosing a range of surface mechanics of biological interest. In osteogenic environment, human mesenchymal stromal cells were more responsive to surface roughness than to surface stiffness. Cells were particularly influenced by overall crystal size distribution, not by average roughness. Absence of mold-imposed boundary constrains makes additive manufacturing ideal to spatially control crystallization and henceforward surface roughness of semi-crystalline thermoplastics. Graphic abstract

Original language	English
Pages (from-to)	3914-3935
Number of pages	22
Journal	Journal of Materials Research
Volume	36
Issue number	19
DOIs	https://doi.org/10.1557/s43578-021-00403-2
Publication status	Published - 14 Oct 2021

Keywords

Crystalline
Surface chemistry
Surface topography
3D printing
Tissue engineering
MESENCHYMAL STEM-CELLS
MATRIX STIFFNESS
MOLECULAR-WEIGHT
DIFFERENTIATION
CRYSTALLINITY
ROUGHNESS
FUNCTIONALIZATION
PROLIFERATION
FABRICATION
ELASTICITY

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Calore, A. R., Srinivas, V., Anand, S., Albillos-Sanchez, A., Looijmans, S. F. S. P., van Breemen, L. C. A., Mota, C., Bernaerts, K., Harings, J. A. W., & Moroni, L. (2021). Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate. Journal of Materials Research, 36(19), 3914-3935. https://doi.org/10.1557/s43578-021-00403-2

@article{787f814250654ceaacf299c2269b6523,

title = "Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate",

abstract = "Thermoplastic semi-crystalline polymers are excellent candidates for tissue engineering scaffolds thanks to facile processing and tunable properties, employed in melt-based additive manufacturing. Control of crystallization and ultimate crystallinity during processing affect properties like surface stiffness and roughness. These in turn influence cell attachment, proliferation and differentiation. Surface stiffness and roughness are intertwined via crystallinity, but never studied independently. The targeted stiffness range is besides difficult to realize for a single thermoplastic. Via correlation of thermal history, crystallization and ultimate crystallinity of vitamin E plasticized poly(lactide), surface stiffness and roughness are decoupled, disclosing a range of surface mechanics of biological interest. In osteogenic environment, human mesenchymal stromal cells were more responsive to surface roughness than to surface stiffness. Cells were particularly influenced by overall crystal size distribution, not by average roughness. Absence of mold-imposed boundary constrains makes additive manufacturing ideal to spatially control crystallization and henceforward surface roughness of semi-crystalline thermoplastics. Graphic abstract",

keywords = "Crystalline, Surface chemistry, Surface topography, 3D printing, Tissue engineering, MESENCHYMAL STEM-CELLS, MATRIX STIFFNESS, MOLECULAR-WEIGHT, DIFFERENTIATION, CRYSTALLINITY, ROUGHNESS, FUNCTIONALIZATION, PROLIFERATION, FABRICATION, ELASTICITY",

author = "A.R. Calore and V. Srinivas and S. Anand and A. Albillos-Sanchez and S.F.S.P. Looijmans and {van Breemen}, L.C.A. and C. Mota and K. Bernaerts and J.A.W. Harings and L. Moroni",

note = "Funding Information: Some of the materials were kindly provided by Corbion (The Netherlands). This work was financed by Brightlands Material Center and by the Dutch Province of Limburg. This study was also supported by the 4NanoEARDRM project, funded under the frame of EuroNanoMed III, an ERA-NET Cofund scheme of the Horizon 2020 Research and Innovation Framework Programme of the European Commission. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = oct,

day = "14",

doi = "10.1557/s43578-021-00403-2",

language = "English",

volume = "36",

pages = "3914--3935",

journal = "Journal of Materials Research",

issn = "0884-2914",

publisher = "Materials Research Society",

number = "19",

}

Calore, AR, Srinivas, V, Anand, S, Albillos-Sanchez, A, Looijmans, SFSP, van Breemen, LCA, Mota, C , Bernaerts, K , Harings, JAW & Moroni, L 2021, 'Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate', Journal of Materials Research, vol. 36, no. 19, pp. 3914-3935. https://doi.org/10.1557/s43578-021-00403-2

Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate. / Calore, A.R.; Srinivas, V.; Anand, S. et al.
In: Journal of Materials Research, Vol. 36, No. 19, 14.10.2021, p. 3914-3935.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate

AU - Calore, A.R.

AU - Srinivas, V.

AU - Anand, S.

AU - Albillos-Sanchez, A.

AU - Looijmans, S.F.S.P.

AU - van Breemen, L.C.A.

AU - Mota, C.

AU - Bernaerts, K.

AU - Harings, J.A.W.

AU - Moroni, L.

N1 - Funding Information: Some of the materials were kindly provided by Corbion (The Netherlands). This work was financed by Brightlands Material Center and by the Dutch Province of Limburg. This study was also supported by the 4NanoEARDRM project, funded under the frame of EuroNanoMed III, an ERA-NET Cofund scheme of the Horizon 2020 Research and Innovation Framework Programme of the European Commission. Publisher Copyright: © 2021, The Author(s).

PY - 2021/10/14

Y1 - 2021/10/14

N2 - Thermoplastic semi-crystalline polymers are excellent candidates for tissue engineering scaffolds thanks to facile processing and tunable properties, employed in melt-based additive manufacturing. Control of crystallization and ultimate crystallinity during processing affect properties like surface stiffness and roughness. These in turn influence cell attachment, proliferation and differentiation. Surface stiffness and roughness are intertwined via crystallinity, but never studied independently. The targeted stiffness range is besides difficult to realize for a single thermoplastic. Via correlation of thermal history, crystallization and ultimate crystallinity of vitamin E plasticized poly(lactide), surface stiffness and roughness are decoupled, disclosing a range of surface mechanics of biological interest. In osteogenic environment, human mesenchymal stromal cells were more responsive to surface roughness than to surface stiffness. Cells were particularly influenced by overall crystal size distribution, not by average roughness. Absence of mold-imposed boundary constrains makes additive manufacturing ideal to spatially control crystallization and henceforward surface roughness of semi-crystalline thermoplastics. Graphic abstract

AB - Thermoplastic semi-crystalline polymers are excellent candidates for tissue engineering scaffolds thanks to facile processing and tunable properties, employed in melt-based additive manufacturing. Control of crystallization and ultimate crystallinity during processing affect properties like surface stiffness and roughness. These in turn influence cell attachment, proliferation and differentiation. Surface stiffness and roughness are intertwined via crystallinity, but never studied independently. The targeted stiffness range is besides difficult to realize for a single thermoplastic. Via correlation of thermal history, crystallization and ultimate crystallinity of vitamin E plasticized poly(lactide), surface stiffness and roughness are decoupled, disclosing a range of surface mechanics of biological interest. In osteogenic environment, human mesenchymal stromal cells were more responsive to surface roughness than to surface stiffness. Cells were particularly influenced by overall crystal size distribution, not by average roughness. Absence of mold-imposed boundary constrains makes additive manufacturing ideal to spatially control crystallization and henceforward surface roughness of semi-crystalline thermoplastics. Graphic abstract

KW - Crystalline

KW - Surface chemistry

KW - Surface topography

KW - 3D printing

KW - Tissue engineering

KW - MESENCHYMAL STEM-CELLS

KW - MATRIX STIFFNESS

KW - MOLECULAR-WEIGHT

KW - DIFFERENTIATION

KW - CRYSTALLINITY

KW - ROUGHNESS

KW - FUNCTIONALIZATION

KW - PROLIFERATION

KW - FABRICATION

KW - ELASTICITY

U2 - 10.1557/s43578-021-00403-2

DO - 10.1557/s43578-021-00403-2

M3 - Article

SN - 0884-2914

VL - 36

SP - 3914

EP - 3935

JO - Journal of Materials Research

JF - Journal of Materials Research

IS - 19

ER -

Shaping and properties of thermoplastic scaffolds in tissue regeneration: The effect of thermal history on polymer crystallization, surface characteristics and cell fate

Abstract

Keywords

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