Serum levels of miR-29, miR-122, miR-155 and miR-192 are elevated in patients with cholangiocarcinoma

Sven H. Loosen, Georg Lurje, Georg Wiltberger, Mihael Vucur, Alexander Koch, Jakob N. Kather, Pia Paffenholz, Frank Tacke, Florian T. Ulmer, Christian Trautwein, Tom Luedde, Ulf P. Neumann*, Christoph Roderburg*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Web of Science)

Abstract

Objectives

Cholangiocarcinoma (CCA) represents the second most common primary hepatic malignancy. Despite tremendous research activities, the prognosis for the majority of patients is still poor. Only in case of early diagnosis, liver resection might potentially lead to long-term survival. However, it is still unclear which patients benefit most from extensive liver surgery, highlighting the need for new diagnostic and prognostic stratification strategies.

Methods

Serum concentrations of a 4 miRNA panel (miR-122, miR-192, miR-29b and miR-155) were analyzed using semi-quantitative reverse-transcriptase PCR in serum samples from 94 patients with cholangiocarcinoma undergoing tumour resection and 40 healthy controls. Results were correlated with clinical data.

Results

Serum concentrations of miR-122, miR-192, miR-29b and miR-155 were significantly elevated in patients with CCA compared to healthy controls or patients with primary sclerosing cholangitis without malignant transformation. Although preoperative levels of these miRNAs were unsuitable as a prognostic marker of survival, a strong postoperative decline of miR-122 serum levels was significantly associated with a favorable patients' prognosis.

Conclusions

Analysis of circulating miRNAs represents a promising tool for the diagnosis of even early stage CCA. A postoperative decline in miRNA serum concentrations might be indicative for a favorable patients' outcome and helpful to identify patients with a good prognosis after extended liver surgery.

Original languageEnglish
Article number0210944
Number of pages17
JournalPLOS ONE
Volume14
Issue number1
DOIs
Publication statusPublished - 17 Jan 2019

Keywords

  • HEPATOCELLULAR-CARCINOMA
  • CIRCULATING MICRORNAS
  • INTRAHEPATIC CHOLANGIOCARCINOMA
  • POTENTIAL BIOMARKERS
  • CANCER
  • EXPRESSION
  • DIAGNOSIS
  • REGULATORS
  • MORTALITY
  • MARKERS

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