Abstract
The demand for meat is expected to exceed production capacity by livestock in the coming decennia. Therefore, cultured beef might be a viable alternative to traditional livestock-derived beef. One of the problems however is the sustainability of cultured beef through the use of fetal bovine serum. We aimed to identify a serum-free medium or a serum-replacement that is as effective as the current method used for culturing bovine myoblasts. Cells were harvested from a female Blanc Bleu Belge cow and myoblasts were subsequently isolated. Cells were cultured in either Advanced DMEM containing 20% FBS and 10% HS or one of the chemically-defined, serum-free media for 6 days. MTS was used as a measure of cell proliferation at day 1, 4 or 6 and microscopic pictures were taken to assess cell morphology. FBM (TM), TesR (TM) and Essential 8 (TM) are commercially available xeno-free media developed for human PSCs and fibroblasts, with the highest potential to sustain bovine myoblast proliferation. Of the supplements tested, XenoFree (TM) and a custom-prepared growth factor mix failed to stimulate cell proliferation. LipoGro (TM) stimulated cell proliferation in some cases but also changed the phenotype of myoblasts to an adipocyte-like phenotype. We conclude that serum-free media stimulate exponential cell expansion, albeit not to the extent of the current growth medium containing up to 30% serum. Further research is needed to investigate whether prolonged cell culture or an adaptation period could further increase cell proliferation.
Original language | English |
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Pages (from-to) | 111–120 |
Number of pages | 10 |
Journal | Cytotechnology |
Volume | 72 |
Issue number | 1 |
Early online date | 28 Dec 2019 |
DOIs | |
Publication status | Published - Feb 2020 |
Keywords
- Myoblasts
- Xeno-free media
- Proliferation
- STEM-CELLS
- CULTURE
- PROLIFERATION
- EXPRESSION