@article{2862171304a54fa28c7555c898a99ffc,
title = "SecScan: a general approach for mapping disulfide bonds in synthetic and recombinant peptides and proteins",
abstract = "Selenocysteine scanning (SecScan) is a novel technique to map disulfide networks in proteins independent of structure-based distance information and mass spectrometry. SecScan applies systematic substitution of single Cys by Sec in combination with NMR spectroscopy for reliable and unambiguous determination of disulfide bond networks.",
keywords = "MU-CONOTOXIN-KIIIA, MASS-SPECTROMETRY, ION MOBILITY, BRIDGES, CONNECTIVITIES, DISSOCIATION, LIGATION, TOOL",
author = "Denisov, {Stepan S.} and Ippel, {Johannes H.} and Mans, {Ben J.} and Ingrid Dijkgraaf and Hackeng, {Tilman M.}",
note = "Funding Information: We acknowledge Drs Kolkman and Janssen (DSM Resolve, Geleen, The Netherlands) for technical assistance in recording 77Se NMR spectra; and Drs Andrew Ellington and Ross Thyer (UT Austin, USA) who generously provided E. coli strains for recombinant production of selenoproteins. This work has been made possible by The Netherlands Organisation for Scientific Research (NWO; VIDI grant 723.013.009 and Aspasia 015.010.005 grant to ID). Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",
year = "2019",
month = feb,
day = "4",
doi = "10.1039/c8cc08777f",
language = "English",
volume = "55",
pages = "1374--1377",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "10",
}