Secondary Ion Mass Spectrometry Imaging of Dictyostelium discoideum Aggregation Streams

John Daniel DeBord; Donald F. Smith; Christopher R. Anderton; Ron M. A. Heeren; Ljiljana Pasa-Tolic; Richard H. Gomer; Francisco A. Fernandez-Lima

doi:10.1371/journal.pone.0099319

Secondary Ion Mass Spectrometry Imaging of Dictyostelium discoideum Aggregation Streams

John Daniel DeBord, Donald F. Smith, Christopher R. Anderton, Ron M. A. Heeren, Ljiljana Pasa-Tolic, Richard H. Gomer, Francisco A. Fernandez-Lima^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Web of Science)

Abstract

High resolution imaging mass spectrometry could become a valuable tool for cell and developmental biology, but both, high spatial and mass spectral resolution are needed to enable this. In this report, we employed Bi3 bombardment time-of-flight (Bi3 ToF-SIMS) and C60 bombardment Fourier transform ion cyclotron resonance secondary ion mass spectrometry (C60 FTICR-SIMS) to image Dictyostelium discoideum aggregation streams. Nearly 300 lipid species were identified from the aggregation streams. High resolution mass spectrometry imaging (FTICR-SIMS) enabled the generation of multiple molecular ion maps at the nominal mass level and provided good coverage for fatty acyls, prenol lipids, and sterol lipids. The comparison of Bi3 ToF-SIMS and C60 FTICR-SIMS suggested that while the first provides fast, high spatial resolution molecular ion images, the chemical complexity of biological samples warrants the use of high resolution analyzers for accurate ion identification.

Original language	English
Journal	PLOS ONE
Volume	9
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0099319
Publication status	Published - 9 Jun 2014

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10.1371/journal.pone.0099319

Cite this

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title = "Secondary Ion Mass Spectrometry Imaging of Dictyostelium discoideum Aggregation Streams",

abstract = "High resolution imaging mass spectrometry could become a valuable tool for cell and developmental biology, but both, high spatial and mass spectral resolution are needed to enable this. In this report, we employed Bi3 bombardment time-of-flight (Bi3 ToF-SIMS) and C60 bombardment Fourier transform ion cyclotron resonance secondary ion mass spectrometry (C60 FTICR-SIMS) to image Dictyostelium discoideum aggregation streams. Nearly 300 lipid species were identified from the aggregation streams. High resolution mass spectrometry imaging (FTICR-SIMS) enabled the generation of multiple molecular ion maps at the nominal mass level and provided good coverage for fatty acyls, prenol lipids, and sterol lipids. The comparison of Bi3 ToF-SIMS and C60 FTICR-SIMS suggested that while the first provides fast, high spatial resolution molecular ion images, the chemical complexity of biological samples warrants the use of high resolution analyzers for accurate ion identification.",

author = "DeBord, {John Daniel} and Smith, {Donald F.} and Anderton, {Christopher R.} and Heeren, {Ron M. A.} and Ljiljana Pasa-Tolic and Gomer, {Richard H.} and Fernandez-Lima, {Francisco A.}",

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AU - DeBord, John Daniel

AU - Smith, Donald F.

AU - Anderton, Christopher R.

AU - Heeren, Ron M. A.

AU - Pasa-Tolic, Ljiljana

AU - Gomer, Richard H.

AU - Fernandez-Lima, Francisco A.

PY - 2014/6/9

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AB - High resolution imaging mass spectrometry could become a valuable tool for cell and developmental biology, but both, high spatial and mass spectral resolution are needed to enable this. In this report, we employed Bi3 bombardment time-of-flight (Bi3 ToF-SIMS) and C60 bombardment Fourier transform ion cyclotron resonance secondary ion mass spectrometry (C60 FTICR-SIMS) to image Dictyostelium discoideum aggregation streams. Nearly 300 lipid species were identified from the aggregation streams. High resolution mass spectrometry imaging (FTICR-SIMS) enabled the generation of multiple molecular ion maps at the nominal mass level and provided good coverage for fatty acyls, prenol lipids, and sterol lipids. The comparison of Bi3 ToF-SIMS and C60 FTICR-SIMS suggested that while the first provides fast, high spatial resolution molecular ion images, the chemical complexity of biological samples warrants the use of high resolution analyzers for accurate ion identification.

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