TY - JOUR
T1 - Risk assessment of the antifungal and insecticidal peptide Jaburetox and its parental protein the Jack bean (Canavalia ensiformis) urease
AU - Sa, Chayenne Alves
AU - Vieira, Leonardo Rogerio
AU - Pereira Almeida Filho, Luiz Carlos
AU - Real-Guerra, Rafael
AU - Lopes, Fernanda Cortez
AU - Souza, Terezinha Maria
AU - Vasconcelos, Ilka Maria
AU - Staniscuaski, Fernanda
AU - Carlini, Celia Regina
AU - Urano Carvalho, Ana Fontenele
AU - Farias, Davi Felipe
N1 - Funding Information:
We thank to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil , for supporting this research with grants (Grant number 461182-9 ) and scholarships.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/2
Y1 - 2020/2
N2 - Jaburetox (JBTX) is an insecticidal and antifungal peptide derived from jack bean (Canavalia ensiformis) urease that has been considered a candidate for developing genetically modified crops. This study aimed to perform the risk assessment of the peptide JBTX following the general recommendations of the two-tiered, weight-of-evidence approach proposed by International Life Sciences Institute. The urease of C. ensiformis (JBU) and its isoform JBURE IIb (the JBTX parental protein) were assessed. The history of safe use revealed no hazard reports for the studied proteins. The available information shows that JBTX possesses selective activity against insects and fungi. JBTX and JBU primary amino acids sequences showed no relevant similarity to toxic, antinutritional or allergenic proteins. Additionally, JBTX and JBU were susceptible to in vitro digestibility, and JBU was also susceptible to heat treatment. The results did not identify potential risks of adverse effects and reactions associated to JBTX. However, further allergen (e.g. serum IgE binding test) and toxicity (e.g. rodent toxicity tests) experimentation can be done to gather additional safety information on JBTX, and to meet regulatory inquiries for commercial approval of transgenic cultivars expressing this peptide.
AB - Jaburetox (JBTX) is an insecticidal and antifungal peptide derived from jack bean (Canavalia ensiformis) urease that has been considered a candidate for developing genetically modified crops. This study aimed to perform the risk assessment of the peptide JBTX following the general recommendations of the two-tiered, weight-of-evidence approach proposed by International Life Sciences Institute. The urease of C. ensiformis (JBU) and its isoform JBURE IIb (the JBTX parental protein) were assessed. The history of safe use revealed no hazard reports for the studied proteins. The available information shows that JBTX possesses selective activity against insects and fungi. JBTX and JBU primary amino acids sequences showed no relevant similarity to toxic, antinutritional or allergenic proteins. Additionally, JBTX and JBU were susceptible to in vitro digestibility, and JBU was also susceptible to heat treatment. The results did not identify potential risks of adverse effects and reactions associated to JBTX. However, further allergen (e.g. serum IgE binding test) and toxicity (e.g. rodent toxicity tests) experimentation can be done to gather additional safety information on JBTX, and to meet regulatory inquiries for commercial approval of transgenic cultivars expressing this peptide.
KW - Allergen prediction
KW - Candidate protein
KW - Food safety assessment
KW - Genetically modified crops
KW - In vitro digestibility
KW - FOOD SAFETY ASSESSMENT
KW - TOXIC PROTEIN
KW - PROTEOLYTIC ACTIVATION
KW - RHODNIUS-PROLIXUS
KW - GENUS CANAVALIA
KW - IN-VITRO
KW - CANATOXIN
KW - EXPRESSION
KW - HEMIPTERA
KW - CHANNELS
U2 - 10.1016/j.fct.2019.110977
DO - 10.1016/j.fct.2019.110977
M3 - Article
C2 - 31759068
SN - 0278-6915
VL - 136
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
M1 - 110977
ER -