Risk assessment of the antifungal and insecticidal peptide Jaburetox and its parental protein the Jack bean (Canavalia ensiformis) urease

Chayenne Alves Sa, Leonardo Rogerio Vieira, Luiz Carlos Pereira Almeida Filho, Rafael Real-Guerra, Fernanda Cortez Lopes, Terezinha Maria Souza, Ilka Maria Vasconcelos, Fernanda Staniscuaski, Celia Regina Carlini, Ana Fontenele Urano Carvalho, Davi Felipe Farias*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Web of Science)

Abstract

Jaburetox (JBTX) is an insecticidal and antifungal peptide derived from jack bean (Canavalia ensiformis) urease that has been considered a candidate for developing genetically modified crops. This study aimed to perform the risk assessment of the peptide JBTX following the general recommendations of the two-tiered, weight-of-evidence approach proposed by International Life Sciences Institute. The urease of C. ensiformis (JBU) and its isoform JBURE IIb (the JBTX parental protein) were assessed. The history of safe use revealed no hazard reports for the studied proteins. The available information shows that JBTX possesses selective activity against insects and fungi. JBTX and JBU primary amino acids sequences showed no relevant similarity to toxic, antinutritional or allergenic proteins. Additionally, JBTX and JBU were susceptible to in vitro digestibility, and JBU was also susceptible to heat treatment. The results did not identify potential risks of adverse effects and reactions associated to JBTX. However, further allergen (e.g. serum IgE binding test) and toxicity (e.g. rodent toxicity tests) experimentation can be done to gather additional safety information on JBTX, and to meet regulatory inquiries for commercial approval of transgenic cultivars expressing this peptide.

Original languageEnglish
Article number110977
Number of pages13
JournalFood and Chemical Toxicology
Volume136
DOIs
Publication statusPublished - Feb 2020

Keywords

  • Allergen prediction
  • Candidate protein
  • Food safety assessment
  • Genetically modified crops
  • In vitro digestibility
  • FOOD SAFETY ASSESSMENT
  • TOXIC PROTEIN
  • PROTEOLYTIC ACTIVATION
  • RHODNIUS-PROLIXUS
  • GENUS CANAVALIA
  • IN-VITRO
  • CANATOXIN
  • EXPRESSION
  • HEMIPTERA
  • CHANNELS

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