TY - JOUR
T1 - Responsiveness to pulmonary rehabilitation in COPD is associated with changes in microbiota
AU - Melo-Dias, S.
AU - Cabral, M.
AU - Furtado, A.
AU - Souto-Miranda, S.
AU - Mendes, M.A.
AU - Cravo, J.
AU - Almeida, C.R.
AU - Marques, A.
AU - Sousa, A.
N1 - Funding Information:
This study is integrated in “PRIME—Pulmonary Rehabilitation and microbiota in exacerbations of COPD”, “GENIAL—Genetic and Clinical markers of COPD trajectory”, and "MicroAgeing—The role of microbiota in ageing", funded by Programa Operacional de Competitividade e Internacionalização—COMPETE, through Fundo Europeu de Desenvolvimento Regional—FEDER (POCI-01-0145-FEDER-007628 and POCI-010145-FEDER-028806), Fundação para a Ciência e Tecnologia—FCT (PTDC/SAU-SER/28806/2017, PTDC/DTP-PIC/2284/2014 and PTDC/BIA-EVL/30212/2017) and under the project UIDB/04501/2020. S. Melo-Dias was supported by Grant SFRH/BD/140908/2018 from FCT. S. Souto-Miranda was supported by Grant SFRH/BD/146134/2019. A. Sousa was funded from national funds through FCT – Fundação para a Ciência e a Tecnologia, I.P., under the Scientific Employment Stimulus—Institutional Call—reference CEECINST/00026/2018.
Funding Information:
We are grateful to all people with COPD who accepted to participate in this study and to the team of Respiratory Research and Rehabilitation Laboratory, School of Health Sciences (Lab3R-ESSUA) for their support. S. Melo-Dias, M. Cabral, A. Furtado, S. Souto-Miranda, J. Cravo, M. A. Mendes, C. R. Almeida, A. Marques, A. Sousa, 2022, “Responsiveness to pulmonary rehabilitation is related with changes in oral microbiota of people with COPD” (Oral communication in 38º Congresso de Pneumologia, November 2022); S. Melo-Dias, M. Cabral, A. Furtado, S. Souto-Miranda, J. Cravo, M. A. Mendes, C. R. Almeida, A. Marques, A. Sousa, 2022, “Pulmonary rehabilitation changes the oral microbiota of people with COPD” (Oral communication in ERS International Congress, September 2022); S. Melo-Dias, M. Cabral, A. Furtado, S. Souto-Miranda, C. R. Almeida, A. Marques, A. Sousa, 2022, “The Effectiveness of pulmonary rehabilitation in COPD is associated with specific shifts in oral microbiota” (Poster presentation in the 7th meeting of International Society for Evolution, Medicine and Public Health, July 2022); A. Furtado, S. Melo-Dias, M. Cabral, A. Marques, A. Sousa, 2021, “The effect of pulmonary rehabilitation in salivary microbiota of people with chronic obstructive pulmonary disease: A longitudinal study” (Poster presentation in IV International Conference—“Microbiota and Health”, 22 October 2021).
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/1/25
Y1 - 2023/1/25
N2 - BackgroundPulmonary Rehabilitation (PR) is one of the most cost-effective therapies for chronic obstructive pulmonary disease (COPD) management. There are, however, people who do not respond to PR and reasons for non-response are mostly unknown. PR is likely to change the airway microbiota and this could play a role in its responsiveness. In this study we have explored the association between PR effectiveness and specific alterations in oral microbiota and inflammation.MethodsA prospective longitudinal study was conducted. Data on exercise capacity, dyspnoea, impact of disease and 418 saliva samples were collected from 76 patients, half of whom participated in a 12-weeks PR programme. Responders and non-responders to PR (dyspnoea, exercise-capacity and impact of disease) were defined based on minimal clinically important differences.ResultsChanges in microbiota, including Prevotella melaninogenica and Streptococcus were observed upon PR. Prevotella, previously found to be depleted in severe COPD, increased during the first month of PR in responders. This increase was negatively correlated with Streptococcus and Lautropia, known to be enriched in severe cases of COPD. Simultaneously, an anti-inflammatory commensal of the respiratory tract, Rothia, correlated strongly and negatively with several pro-inflammatory markers, whose levels were generally boosted by PR. Conversely, in non-responders, the observed decline in Prevotella correlated negatively with Streptococcus and Lautropia whose fluctuations co-occurred with several pro-inflammatory markers.ConclusionsPR is associated with changes in oral microbiota. Specifically, PR increases salivary Prevotella melaninogenica and avoids the decline in Rothia and the increase in Streptococcus and Lautropia in responders, which may contribute to the benefits of PR.
AB - BackgroundPulmonary Rehabilitation (PR) is one of the most cost-effective therapies for chronic obstructive pulmonary disease (COPD) management. There are, however, people who do not respond to PR and reasons for non-response are mostly unknown. PR is likely to change the airway microbiota and this could play a role in its responsiveness. In this study we have explored the association between PR effectiveness and specific alterations in oral microbiota and inflammation.MethodsA prospective longitudinal study was conducted. Data on exercise capacity, dyspnoea, impact of disease and 418 saliva samples were collected from 76 patients, half of whom participated in a 12-weeks PR programme. Responders and non-responders to PR (dyspnoea, exercise-capacity and impact of disease) were defined based on minimal clinically important differences.ResultsChanges in microbiota, including Prevotella melaninogenica and Streptococcus were observed upon PR. Prevotella, previously found to be depleted in severe COPD, increased during the first month of PR in responders. This increase was negatively correlated with Streptococcus and Lautropia, known to be enriched in severe cases of COPD. Simultaneously, an anti-inflammatory commensal of the respiratory tract, Rothia, correlated strongly and negatively with several pro-inflammatory markers, whose levels were generally boosted by PR. Conversely, in non-responders, the observed decline in Prevotella correlated negatively with Streptococcus and Lautropia whose fluctuations co-occurred with several pro-inflammatory markers.ConclusionsPR is associated with changes in oral microbiota. Specifically, PR increases salivary Prevotella melaninogenica and avoids the decline in Rothia and the increase in Streptococcus and Lautropia in responders, which may contribute to the benefits of PR.
KW - Oral microbiota
KW - Inflammation
KW - COPD
KW - Pulmonary rehabilitation
KW - Responsiveness
KW - IMPORTANT DIFFERENCE
U2 - 10.1186/s12931-023-02339-z
DO - 10.1186/s12931-023-02339-z
M3 - Article
C2 - 36698137
SN - 1465-9921
VL - 24
JO - Respiratory Research
JF - Respiratory Research
IS - 1
M1 - 29
ER -