Reshaping Lipid Biochemistry by Pushing Barriers in Structural Lipidomics

Tiffany Porta Siegel, Kim Ekroos, Shane R. Ellis*

*Corresponding author for this work

Research output: Contribution to journal(Systematic) Review article peer-review

57 Citations (Web of Science)

Abstract

Lipidomics is a rapidly growing field with numerous examples showing the importance of lipid molecules throughout biology. It has also shed light onto the vast and complex functions performed by many lipids that possess an immense diversity in molecular structures. Mass spectrometry (MS) is the tool of choice for analyzing lipids and has been the key catalyst driving the field forward. However, MS does not yet permit true molecular lipidomics wherein the identification and quantification of lipids having defined molecular structures can be routinely achieved. Here we describe recent advances in MS-based lipidomics that allow access to higher levels of molecular information in lipidomics experiments. These advances will form a key piece of the puzzle as the field moves towards systems characterization of lipids at the molecular level.

Original languageEnglish
Pages (from-to)6492-6501
Number of pages10
JournalAngewandte Chemie-International Edition
Volume58
Issue number20
DOIs
Publication statusPublished - 13 May 2019

Keywords

  • biochemistry
  • isomers
  • lipidomics
  • mass spectrometry
  • structural identification
  • OZONE-INDUCED DISSOCIATION
  • NM ULTRAVIOLET PHOTODISSOCIATION
  • MOBILITY-MASS-SPECTROMETRY
  • DOUBLE-BOND POSITION
  • ACYL-CHAIN POSITION
  • ELECTROSPRAY-IONIZATION
  • LIQUID-CHROMATOGRAPHY
  • QUANTITATIVE-ANALYSIS
  • SHOTGUN LIPIDOMICS
  • MEMBRANE-LIPIDS

Cite this