Reproducibility of Retinal Microvascular Traits Decoded by the Singapore I Vessel Assessment Software Across the Human Age Range

Qi-Fang Huang, Fang-Fei Wei, Zhen-Yu Zhang, Anke Raaijmakers, Kei Asayama, Lutgarde Thijs, Wen-Yi Yang, Blerim Mujaj, Karel Allegaert, Peter Verhamme, Harry A. J. Struijker-Boudier, Yan Li, Jan A. Staessen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Web of Science)

Abstract

BACKGROUND Retinal microvascular traits predict adverse health outcomes. The Singapore I Vessel Assessment (SIVA) software improved automated postprocessing of retinal photographs. In addition to microvessel caliber, it generates measures of arteriolar and venular geometry. Few studies addressed the reproducibility of SIVA measurements across a wide age range. METHODS In the current study, 2 blinded graders read images obtained by non-mydriatic retinal photography twice in 20 11-year-old children, born prematurely (n = 10) or at term (n = 10) and in 60 adults (age range, 18.9-86.1 years). RESULTS Former preterm compared with term children had lower microvessel diameter and disorganized vessel geometry with no differences in intraobserver and interobserver variability. Among adults, microvessel caliber decreased with age and blood pressure and arteriolar geometry was inversely correlated with female sex and age. Intraobserver differences estimated by the Bland-Altman method did not reach significance for any measurement. Across measurements, median reproducibility (RM) expressed as percent of the average trait value was 8.8% in children (median intraclass correlation coefficient [ICC], 0.94) and 8.0% (0.97) in adults. Likewise, interobserver differences did not reach significance with RM (ICC) of 10.6% (0.85) in children and 10.4% (0.93) in adults. Reproducibility was best for microvessel caliber (intraobserver/interobserver RM, 4.7%/6.0%; ICC, 0.98/0.96), worst for venular geometry (17.0%/18.8%; 0.93/0.84), and intermediate for arteriolar geometry (10.9%/14.9%; 0.95/0.86). CONCLUSIONS SIVA produces repeatable measures of the retinal microvasculature in former preterm and term children and in adults, thereby proving its usability from childhood to old age.
Original languageEnglish
Pages (from-to)438-449
Number of pages12
JournalAmerican Journal of Hypertension
Volume31
Issue number4
DOIs
Publication statusPublished - Apr 2018

Keywords

  • blood pressure
  • hypertension
  • microcirculation
  • population research
  • reproducibility
  • retina
  • vascular geometry
  • BLOOD-PRESSURE
  • ATHEROSCLEROSIS RISK
  • ARTERIOLAR DIAMETER
  • VASCULAR CALIBER
  • HYPERTENSION
  • BIRTH
  • GROWTH
  • ABNORMALITIES
  • COMMUNITIES
  • POPULATION

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