Repetitive disruptions of the nuclear envelope invoke temporary loss of cellular compartmentalization in laminopathies

Winnok H. De Vos, Frederik Houben, Miriam Kamps, Ashraf Malhas, Fons Verheyen, Julien Cox, Erik M. M. Manders, Valerie L. R. M. Verstraeten, Maurice A. M. van Steensel, Carlo L. M. Marcelis, Arthur van den Wijngaard, David J. Vaux, Frans C. S. Ramaekers, Jos L. V. Broers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


The nuclear lamina provides structural support to the nucleus and has a central role in nuclear organization and gene regulation. Defects in its constituents, the lamins, lead to a class of genetic diseases collectively referred to as laminopathies. Using live cell imaging, we observed the occurrence of intermittent, non-lethal ruptures of the nuclear envelope in dermal fibroblast cultures of patients with different mutations of lamin A/C. These ruptures, which were absent in normal fibroblasts, could be mimicked by selective knockdown as well as knockout of LMNA and were accompanied by the loss of cellular compartmentalization. This was demonstrated by the influx of cytoplasmic transcription factor RelA and regulatory protein Cyclin B1 into the nucleus, and efflux of nuclear transcription factor OCT1 and nuclear structures containing the promyelocytic leukemia (PML) tumour suppressor protein to the cytoplasm. While recovery of enhanced yellow fluorescent protein-tagged nuclear localization signal in the nucleus demonstrated restoration of nuclear membrane integrity, part of the mobile PML structures became permanently translocated to the cytoplasm. These satellite PML structures were devoid of the typical PML body components, such as DAXX, SP100 or SUMO1. Our data suggest that nuclear rupture and loss of compartmentalization may add to cellular dysfunction and disease development in various laminopathies.
Original languageEnglish
Pages (from-to)4175-4186
JournalHuman Molecular Genetics
Issue number21
Publication statusPublished - 1 Nov 2011

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