Refined control of CRISPR-Cas9 gene editing in Clostridium sporogenes: the creation of recombinant strains for therapeutic applications

Keyphrases

• Clostridium Sporogenes 100%
• Cas9 Protein 100%
• Therapeutic Use 100%
• Recombinant Strain 100%
• Gene Editing 100%
• Plasmid 66%
• Cytokine Activity 66%
• Inflammatory Cytokines 33%
• Tumor Tissue 33%
• Healthy Tissue 33%
• Granulocyte Colony-stimulating Factor (G-CSF) 33%
• Conjugation 33%
• Prodrug 33%
• In Vivo Analysis 33%
• Substantial Reduction 33%
• Cell-based 33%
• Interleukin-2 33%
• Clinical Efficacy 33%
• Effective Dose 33%
• Functional Assay 33%
• Gene Knockout 33%
• Genome Editing 33%
• Cancer Immunotherapy 33%
• Knock 33%
• Genetic Engineering 33%
• Converting Enzyme 33%
• Tumor Homing 33%
• Systemic Delivery 33%
• Immune-related Adverse Events 33%
• Operon 33%
• Therapeutic Gene 33%
• Inducible Promoter 33%
• Therapeutic Antibody 33%
• Parental Strain 33%
• Tetracycline-inducible 33%
• Guide RNA (gRNA) 33%
• Fold Increase 33%
• Number of Patients 33%
• Pre-existing Autoimmune Disease 33%
• Tumor Targeting Strategy 33%
• Large Sequences 33%

INIS

• applications 100%
• control 100%
• genes 100%
• strains 100%
• clostridium 100%
• cytokines 57%
• tumors 42%
• antibodies 28%
• patients 28%
• cancer 28%
• tissues 28%
• plasmids 28%
• diseases 14%
• levels 14%
• reduction 14%
• drugs 14%
• tools 14%
• comparative evaluations 14%
• efficiency 14%
• delivery 14%
• losses 14%
• oxygen 14%
• macrophages 14%
• inflammation 14%
• proteins 14%
• enzymes 14%
• vectors 14%
• in vitro 14%
• utilities 14%
• bacteria 14%
• spores 14%
• chromosomes 14%
• interleukins 14%
• immunotherapy 14%
• granulocytes 14%
• promoters 14%
• knock-out reactions 14%
• colonies 14%
• effective doses 14%
• cloning 14%
• tetracyclines 14%

Immunology and Microbiology

• Cytokine 100%
• Clostridium sporogenes 100%
• CRISPR/Cas9 100%
• Gene Editing 100%
• Plasmid 66%
• Promoter Region 33%
• Proinflammatory Cytokine 33%
• Operon 33%
• Autoimmune Disease 33%
• Immunity 33%
• Granulocyte Macrophage Colony-Stimulating Factor 33%
• Interleukin 2 33%
• Gene Expression System 33%
• Cancer Immunotherapy 33%
• Therapeutic Antibodies 33%
• Immunology 33%