Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie Ménard apples, rich in polyphenols.

C. Castagnini, C. Luceri, S. Toti, E. Bigagli, G. Caderni, A.P. Femia, L. Giovannelli, M. Lodovici, V. Pitozzi, M. Salvadori, L. Messerini, R. Martin, E.G. Zoetendal, S. Gaj, L.M. Eijssen, C.T. Evelo, C.M. Renard, A. Baron, P. Dolara

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Abstract

Inflammatory bowel diseases (IBD) are immunomediated ailments affecting millions of individuals. Although diet is regarded as an important factor influencing IBD, there are no accepted dietary recommendations presently available. We administered 7.6 % lyophilised apples obtained from two cultivars (Golden Delicious and Marie Menard, low and high in polyphenols, respectively) to HLA-B27 transgenic rats which develop spontaneous IBD. After 3 months feeding, rats fed Marie Menard apples had reduced myeloperoxidase activity (3.6 (sem 0.3) v. 2.2 (sem 0.2) U/g tissue; P < 0.05) and reduced cyclo-oxygenase-2 (P < 0.05) and inducible NO synthase gene expression (P < 0.01) in the colon mucosa and significantly less diarrhoea (P < 0.05), compared with control rats. Cell proliferation in the colon mucosa was reduced significantly by feeding Golden Delicious apples, with a borderline effect of Marie Menard apples. Gene expression profiling of the colon mucosa, analysed using the Whole Rat Genome 4 x 44 K Agilent Arrays, revealed a down-regulation of the pathways of PG synthesis, mitogen-activated protein kinase (MAPK) signalling and TNFalpha-NF-kappaB in Marie Menard-fed rats. In the stools of the animals of this group we also measured a significant reduction of bacteria of the Bacteriodes fragilis group. In conclusion, the administration of Marie Menard apples, rich in polyphenols and used at present only in the manufacturing of cider, ameliorates colon inflammation in transgenic rats developing spontaneous intestinal inflammation, suggesting the possible use of these and other apple varieties to control inflammation in IBD patients.
Original languageEnglish
Pages (from-to)1620-1628
JournalBritish Journal of Nutrition
Volume102
Issue number11
DOIs
Publication statusPublished - 1 Jan 2009

Cite this

Castagnini, C., Luceri, C., Toti, S., Bigagli, E., Caderni, G., Femia, A. P., ... Dolara, P. (2009). Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie Ménard apples, rich in polyphenols. British Journal of Nutrition, 102(11), 1620-1628. https://doi.org/10.1017/S0007114509990936
Castagnini, C. ; Luceri, C. ; Toti, S. ; Bigagli, E. ; Caderni, G. ; Femia, A.P. ; Giovannelli, L. ; Lodovici, M. ; Pitozzi, V. ; Salvadori, M. ; Messerini, L. ; Martin, R. ; Zoetendal, E.G. ; Gaj, S. ; Eijssen, L.M. ; Evelo, C.T. ; Renard, C.M. ; Baron, A. ; Dolara, P. / Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie Ménard apples, rich in polyphenols. In: British Journal of Nutrition. 2009 ; Vol. 102, No. 11. pp. 1620-1628.
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title = "Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie M{\'e}nard apples, rich in polyphenols.",
abstract = "Inflammatory bowel diseases (IBD) are immunomediated ailments affecting millions of individuals. Although diet is regarded as an important factor influencing IBD, there are no accepted dietary recommendations presently available. We administered 7.6 {\%} lyophilised apples obtained from two cultivars (Golden Delicious and Marie Menard, low and high in polyphenols, respectively) to HLA-B27 transgenic rats which develop spontaneous IBD. After 3 months feeding, rats fed Marie Menard apples had reduced myeloperoxidase activity (3.6 (sem 0.3) v. 2.2 (sem 0.2) U/g tissue; P < 0.05) and reduced cyclo-oxygenase-2 (P < 0.05) and inducible NO synthase gene expression (P < 0.01) in the colon mucosa and significantly less diarrhoea (P < 0.05), compared with control rats. Cell proliferation in the colon mucosa was reduced significantly by feeding Golden Delicious apples, with a borderline effect of Marie Menard apples. Gene expression profiling of the colon mucosa, analysed using the Whole Rat Genome 4 x 44 K Agilent Arrays, revealed a down-regulation of the pathways of PG synthesis, mitogen-activated protein kinase (MAPK) signalling and TNFalpha-NF-kappaB in Marie Menard-fed rats. In the stools of the animals of this group we also measured a significant reduction of bacteria of the Bacteriodes fragilis group. In conclusion, the administration of Marie Menard apples, rich in polyphenols and used at present only in the manufacturing of cider, ameliorates colon inflammation in transgenic rats developing spontaneous intestinal inflammation, suggesting the possible use of these and other apple varieties to control inflammation in IBD patients.",
author = "C. Castagnini and C. Luceri and S. Toti and E. Bigagli and G. Caderni and A.P. Femia and L. Giovannelli and M. Lodovici and V. Pitozzi and M. Salvadori and L. Messerini and R. Martin and E.G. Zoetendal and S. Gaj and L.M. Eijssen and C.T. Evelo and C.M. Renard and A. Baron and P. Dolara",
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Castagnini, C, Luceri, C, Toti, S, Bigagli, E, Caderni, G, Femia, AP, Giovannelli, L, Lodovici, M, Pitozzi, V, Salvadori, M, Messerini, L, Martin, R, Zoetendal, EG, Gaj, S, Eijssen, LM, Evelo, CT, Renard, CM, Baron, A & Dolara, P 2009, 'Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie Ménard apples, rich in polyphenols.', British Journal of Nutrition, vol. 102, no. 11, pp. 1620-1628. https://doi.org/10.1017/S0007114509990936

Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie Ménard apples, rich in polyphenols. / Castagnini, C.; Luceri, C.; Toti, S.; Bigagli, E.; Caderni, G.; Femia, A.P.; Giovannelli, L.; Lodovici, M.; Pitozzi, V.; Salvadori, M.; Messerini, L.; Martin, R.; Zoetendal, E.G.; Gaj, S.; Eijssen, L.M.; Evelo, C.T.; Renard, C.M.; Baron, A.; Dolara, P.

In: British Journal of Nutrition, Vol. 102, No. 11, 01.01.2009, p. 1620-1628.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Reduction of colonic inflammation in HLA-B27 transgenic rats by feeding Marie Ménard apples, rich in polyphenols.

AU - Castagnini, C.

AU - Luceri, C.

AU - Toti, S.

AU - Bigagli, E.

AU - Caderni, G.

AU - Femia, A.P.

AU - Giovannelli, L.

AU - Lodovici, M.

AU - Pitozzi, V.

AU - Salvadori, M.

AU - Messerini, L.

AU - Martin, R.

AU - Zoetendal, E.G.

AU - Gaj, S.

AU - Eijssen, L.M.

AU - Evelo, C.T.

AU - Renard, C.M.

AU - Baron, A.

AU - Dolara, P.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Inflammatory bowel diseases (IBD) are immunomediated ailments affecting millions of individuals. Although diet is regarded as an important factor influencing IBD, there are no accepted dietary recommendations presently available. We administered 7.6 % lyophilised apples obtained from two cultivars (Golden Delicious and Marie Menard, low and high in polyphenols, respectively) to HLA-B27 transgenic rats which develop spontaneous IBD. After 3 months feeding, rats fed Marie Menard apples had reduced myeloperoxidase activity (3.6 (sem 0.3) v. 2.2 (sem 0.2) U/g tissue; P < 0.05) and reduced cyclo-oxygenase-2 (P < 0.05) and inducible NO synthase gene expression (P < 0.01) in the colon mucosa and significantly less diarrhoea (P < 0.05), compared with control rats. Cell proliferation in the colon mucosa was reduced significantly by feeding Golden Delicious apples, with a borderline effect of Marie Menard apples. Gene expression profiling of the colon mucosa, analysed using the Whole Rat Genome 4 x 44 K Agilent Arrays, revealed a down-regulation of the pathways of PG synthesis, mitogen-activated protein kinase (MAPK) signalling and TNFalpha-NF-kappaB in Marie Menard-fed rats. In the stools of the animals of this group we also measured a significant reduction of bacteria of the Bacteriodes fragilis group. In conclusion, the administration of Marie Menard apples, rich in polyphenols and used at present only in the manufacturing of cider, ameliorates colon inflammation in transgenic rats developing spontaneous intestinal inflammation, suggesting the possible use of these and other apple varieties to control inflammation in IBD patients.

AB - Inflammatory bowel diseases (IBD) are immunomediated ailments affecting millions of individuals. Although diet is regarded as an important factor influencing IBD, there are no accepted dietary recommendations presently available. We administered 7.6 % lyophilised apples obtained from two cultivars (Golden Delicious and Marie Menard, low and high in polyphenols, respectively) to HLA-B27 transgenic rats which develop spontaneous IBD. After 3 months feeding, rats fed Marie Menard apples had reduced myeloperoxidase activity (3.6 (sem 0.3) v. 2.2 (sem 0.2) U/g tissue; P < 0.05) and reduced cyclo-oxygenase-2 (P < 0.05) and inducible NO synthase gene expression (P < 0.01) in the colon mucosa and significantly less diarrhoea (P < 0.05), compared with control rats. Cell proliferation in the colon mucosa was reduced significantly by feeding Golden Delicious apples, with a borderline effect of Marie Menard apples. Gene expression profiling of the colon mucosa, analysed using the Whole Rat Genome 4 x 44 K Agilent Arrays, revealed a down-regulation of the pathways of PG synthesis, mitogen-activated protein kinase (MAPK) signalling and TNFalpha-NF-kappaB in Marie Menard-fed rats. In the stools of the animals of this group we also measured a significant reduction of bacteria of the Bacteriodes fragilis group. In conclusion, the administration of Marie Menard apples, rich in polyphenols and used at present only in the manufacturing of cider, ameliorates colon inflammation in transgenic rats developing spontaneous intestinal inflammation, suggesting the possible use of these and other apple varieties to control inflammation in IBD patients.

U2 - 10.1017/S0007114509990936

DO - 10.1017/S0007114509990936

M3 - Article

VL - 102

SP - 1620

EP - 1628

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 11

ER -