Reduced incorporation of Fatty acids into triacylglycerol in myotubes from obese individuals with type 2 diabetes.

L.M. Sparks, M. Bosma, B. Brouwers, T. van de Weijer, L. Bilet, G. Schaart, E. Kornips, T.O. Eichmann, A. Lass, M.K. Hesselink, P. Schrauwen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Altered skeletal muscle lipid metabolism is a hallmark feature of type 2 (T2D). Here we investigated muscle lipid turnover in T2D versus BMI- controls and examined if putative in vivo differences would be preserved myotubes.Male obese T2D individuals (T2D) (n=6) and their BMI-matched (C) (n=6) underwent a hyperinsulinemic-euglycemic clamp, VO2max test, underwater weighing and muscle biopsy of v. lateralis. 14C-palmitate and 14C-oleate oxidation rates and incorporation into lipids were measured tissue, as well as in primary myotubes.Palmitate oxidation (C: 0.99 +/- T2D: 0.53 +/- 0.07nmol/mg protein; P=0.03) and incorporation of fatty into triacylglycerol (TAG) (C: 0.45 +/- 0.13, T2D: 0.11 +/- 0.02nmol/mg P=0.047) were significantly reduced in muscle homogenates of T2D. These reductions were not retained for palmitate oxidation in primary myotubes (P=0.38); however, incorporation of FAs into TAG was lower in T2D oleate and P=0.11 for palmitate), with a strong correlation of TAG between muscle tissue and primary myotubes (r=0.848, P=0.008).Our data that the ability to incorporate FAs into TAG is an intrinsic feature of muscle cells that is reduced in individuals with T2D.
Original languageEnglish
Pages (from-to)1583-1593
Number of pages11
JournalDiabetes
Volume63
Issue number5
DOIs
Publication statusPublished - May 2014

Keywords

  • MUSCLE INSULIN-RESISTANCE
  • SKELETAL-MUSCLE
  • LIPID-METABOLISM
  • SENSITIVITY
  • HUMANS
  • EXPRESSION
  • OXIDATION
  • GLUCOSE
  • QUANTIFICATION
  • SPECTROSCOPY

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