Reduced incorporation of Fatty acids into triacylglycerol in myotubes from obese individuals with type 2 diabetes.

L.M. Sparks, M. Bosma, B. Brouwers, T. van de Weijer, L. Bilet, G. Schaart, E. Kornips, T.O. Eichmann, A. Lass, M.K. Hesselink, P. Schrauwen

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Altered skeletal muscle lipid metabolism is a hallmark feature of type 2 (T2D). Here we investigated muscle lipid turnover in T2D versus BMI- controls and examined if putative in vivo differences would be preserved myotubes.Male obese T2D individuals (T2D) (n=6) and their BMI-matched (C) (n=6) underwent a hyperinsulinemic-euglycemic clamp, VO2max test, underwater weighing and muscle biopsy of v. lateralis. 14C-palmitate and 14C-oleate oxidation rates and incorporation into lipids were measured tissue, as well as in primary myotubes.Palmitate oxidation (C: 0.99 +/- T2D: 0.53 +/- 0.07nmol/mg protein; P=0.03) and incorporation of fatty into triacylglycerol (TAG) (C: 0.45 +/- 0.13, T2D: 0.11 +/- 0.02nmol/mg P=0.047) were significantly reduced in muscle homogenates of T2D. These reductions were not retained for palmitate oxidation in primary myotubes (P=0.38); however, incorporation of FAs into TAG was lower in T2D oleate and P=0.11 for palmitate), with a strong correlation of TAG between muscle tissue and primary myotubes (r=0.848, P=0.008).Our data that the ability to incorporate FAs into TAG is an intrinsic feature of muscle cells that is reduced in individuals with T2D.
Original languageEnglish
Pages (from-to)1583-1593
Number of pages11
JournalDiabetes
Volume63
Issue number5
DOIs
Publication statusPublished - May 2014

Keywords

  • MUSCLE INSULIN-RESISTANCE
  • SKELETAL-MUSCLE
  • LIPID-METABOLISM
  • SENSITIVITY
  • HUMANS
  • EXPRESSION
  • OXIDATION
  • GLUCOSE
  • QUANTIFICATION
  • SPECTROSCOPY

Cite this

@article{343ec50504274df0be463c149ba1b0ae,
title = "Reduced incorporation of Fatty acids into triacylglycerol in myotubes from obese individuals with type 2 diabetes.",
abstract = "Altered skeletal muscle lipid metabolism is a hallmark feature of type 2 (T2D). Here we investigated muscle lipid turnover in T2D versus BMI- controls and examined if putative in vivo differences would be preserved myotubes.Male obese T2D individuals (T2D) (n=6) and their BMI-matched (C) (n=6) underwent a hyperinsulinemic-euglycemic clamp, VO2max test, underwater weighing and muscle biopsy of v. lateralis. 14C-palmitate and 14C-oleate oxidation rates and incorporation into lipids were measured tissue, as well as in primary myotubes.Palmitate oxidation (C: 0.99 +/- T2D: 0.53 +/- 0.07nmol/mg protein; P=0.03) and incorporation of fatty into triacylglycerol (TAG) (C: 0.45 +/- 0.13, T2D: 0.11 +/- 0.02nmol/mg P=0.047) were significantly reduced in muscle homogenates of T2D. These reductions were not retained for palmitate oxidation in primary myotubes (P=0.38); however, incorporation of FAs into TAG was lower in T2D oleate and P=0.11 for palmitate), with a strong correlation of TAG between muscle tissue and primary myotubes (r=0.848, P=0.008).Our data that the ability to incorporate FAs into TAG is an intrinsic feature of muscle cells that is reduced in individuals with T2D.",
keywords = "MUSCLE INSULIN-RESISTANCE, SKELETAL-MUSCLE, LIPID-METABOLISM, SENSITIVITY, HUMANS, EXPRESSION, OXIDATION, GLUCOSE, QUANTIFICATION, SPECTROSCOPY",
author = "L.M. Sparks and M. Bosma and B. Brouwers and {van de Weijer}, T. and L. Bilet and G. Schaart and E. Kornips and T.O. Eichmann and A. Lass and M.K. Hesselink and P. Schrauwen",
year = "2014",
month = "5",
doi = "10.2337/db13-1123",
language = "English",
volume = "63",
pages = "1583--1593",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association",
number = "5",

}

Reduced incorporation of Fatty acids into triacylglycerol in myotubes from obese individuals with type 2 diabetes. / Sparks, L.M.; Bosma, M.; Brouwers, B.; van de Weijer, T.; Bilet, L.; Schaart, G.; Kornips, E.; Eichmann, T.O.; Lass, A.; Hesselink, M.K.; Schrauwen, P.

In: Diabetes, Vol. 63, No. 5, 05.2014, p. 1583-1593.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Reduced incorporation of Fatty acids into triacylglycerol in myotubes from obese individuals with type 2 diabetes.

AU - Sparks, L.M.

AU - Bosma, M.

AU - Brouwers, B.

AU - van de Weijer, T.

AU - Bilet, L.

AU - Schaart, G.

AU - Kornips, E.

AU - Eichmann, T.O.

AU - Lass, A.

AU - Hesselink, M.K.

AU - Schrauwen, P.

PY - 2014/5

Y1 - 2014/5

N2 - Altered skeletal muscle lipid metabolism is a hallmark feature of type 2 (T2D). Here we investigated muscle lipid turnover in T2D versus BMI- controls and examined if putative in vivo differences would be preserved myotubes.Male obese T2D individuals (T2D) (n=6) and their BMI-matched (C) (n=6) underwent a hyperinsulinemic-euglycemic clamp, VO2max test, underwater weighing and muscle biopsy of v. lateralis. 14C-palmitate and 14C-oleate oxidation rates and incorporation into lipids were measured tissue, as well as in primary myotubes.Palmitate oxidation (C: 0.99 +/- T2D: 0.53 +/- 0.07nmol/mg protein; P=0.03) and incorporation of fatty into triacylglycerol (TAG) (C: 0.45 +/- 0.13, T2D: 0.11 +/- 0.02nmol/mg P=0.047) were significantly reduced in muscle homogenates of T2D. These reductions were not retained for palmitate oxidation in primary myotubes (P=0.38); however, incorporation of FAs into TAG was lower in T2D oleate and P=0.11 for palmitate), with a strong correlation of TAG between muscle tissue and primary myotubes (r=0.848, P=0.008).Our data that the ability to incorporate FAs into TAG is an intrinsic feature of muscle cells that is reduced in individuals with T2D.

AB - Altered skeletal muscle lipid metabolism is a hallmark feature of type 2 (T2D). Here we investigated muscle lipid turnover in T2D versus BMI- controls and examined if putative in vivo differences would be preserved myotubes.Male obese T2D individuals (T2D) (n=6) and their BMI-matched (C) (n=6) underwent a hyperinsulinemic-euglycemic clamp, VO2max test, underwater weighing and muscle biopsy of v. lateralis. 14C-palmitate and 14C-oleate oxidation rates and incorporation into lipids were measured tissue, as well as in primary myotubes.Palmitate oxidation (C: 0.99 +/- T2D: 0.53 +/- 0.07nmol/mg protein; P=0.03) and incorporation of fatty into triacylglycerol (TAG) (C: 0.45 +/- 0.13, T2D: 0.11 +/- 0.02nmol/mg P=0.047) were significantly reduced in muscle homogenates of T2D. These reductions were not retained for palmitate oxidation in primary myotubes (P=0.38); however, incorporation of FAs into TAG was lower in T2D oleate and P=0.11 for palmitate), with a strong correlation of TAG between muscle tissue and primary myotubes (r=0.848, P=0.008).Our data that the ability to incorporate FAs into TAG is an intrinsic feature of muscle cells that is reduced in individuals with T2D.

KW - MUSCLE INSULIN-RESISTANCE

KW - SKELETAL-MUSCLE

KW - LIPID-METABOLISM

KW - SENSITIVITY

KW - HUMANS

KW - EXPRESSION

KW - OXIDATION

KW - GLUCOSE

KW - QUANTIFICATION

KW - SPECTROSCOPY

U2 - 10.2337/db13-1123

DO - 10.2337/db13-1123

M3 - Article

VL - 63

SP - 1583

EP - 1593

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 5

ER -