Reduced dietary intake of micronutrients with antioxidant properties negatively impacts muscle health in aged mice

Miriam van Dijk*, Francina J. Dijk, Anita Hartog, Klaske van Norren, Sjors Verlaan, Ardy van Helvoort, Richard T. Jaspers, Yvette Luiking

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Web of Science)

Abstract

Background Inadequate intake of micronutrients with antioxidant properties is common among older adults and has been associated with higher risk of frailty, adverse functional outcome, and impaired muscle health. However, a causal relationship is less well known. The aim was to determine in old mice the impact of reduced dietary intake of vitamins A/E/B6/B12/folate, selenium, and zinc on muscle mass, oxidative capacity, strength, and physical activity (PA) over time.

Methods Twenty-one-month-old male mice were fed either AIN-93-M (control) or a diet low in micronutrients with antioxidant properties (= LOWOX-B: 50% of mouse recommended daily intake of vitamins A, E, B6, and B12, folate, selenium, and zinc) for 4 months. Muscle mass, grip strength, physical activity (PA), and general oxidative status were assessed. Moreover, muscle fatigue was measured of m. extensor digitorum longus (EDL) during an ex vivo moderate exercise protocol. Effects on oxidative capacity [succinate dehydrogenase (SDH) activity], muscle fibre type, number, and fibre cross-sectional area (fCSA) were assessed on m. plantaris (PL) using histochemistry.

Results After 2 months on the diet, bodyweight of LOWOX-B mice was lower compared with control (P <0.0001), mainly due to lower fat mass (P <0.0001), without significant differences in food intake. After 4 months, oxidative status of LOWOX-B mice was lower, demonstrated by decreased vitamin E plasma levels (P <0.05) and increased liver malondialdehyde levels (P = 0.018). PA was lower in LOWOX-B mice (P <0.001 vs. control). Muscle mass was not affected, although PL-fCSA was decreased (similar to 16%; P = 0.028 vs. control). SDH activity and muscle fibre type distribution remained unaffected. In LOWOX-B mice, EDL force production was decreased by 49.7% at lower stimulation frequencies (P = 0.038), and fatigue resistance was diminished (P = 0.023) compared with control.

Conclusions Reduced dietary intake of vitamins A, E, B6, and B12, folate, selenium, and zinc resulted in a lower oxidative capacity and has major impact on muscle health as shown by decreased force production and PA, without effects on muscle mass. The reduced fCSA in combination with similar SDH activity per fibre might explain the reduced oxidative capacity resulting in the increased fatigue after exercise in LOWOX-B mice.

Original languageEnglish
Pages (from-to)146-159
Number of pages14
JournalJournal of cachexia, sarcopenia and muscle
Volume9
Issue number1
DOIs
Publication statusPublished - Feb 2018

Keywords

  • Nutrition
  • Antioxidants
  • Muscle quality
  • Function
  • Strength
  • UBIQUITIN-CONJUGATING ENZYME
  • RAT SOLEUS MUSCLE
  • E3 LIGASE MURF1
  • SKELETAL-MUSCLE
  • RING-FINGER
  • DEPENDENT PROTEOLYSIS
  • PROTEIN LIGASES
  • GENE-EXPRESSION
  • E2 ENZYMES
  • TELETHONIN

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