Real-time sensing of osteoclast activity by electrical impedance

Alexander P.M. Guttenplan; Marijn Lemmens; Gilles Oudebrouckx; Daniel de Melo Pereira; Hoon Suk Rho; Zeinab Tahmasebi Birgani; Stefan Giselbrecht; Roman K. Truckenmüller; Ronald Thoelen; Pamela Habibovic

Real-time sensing of osteoclast activity by electrical impedance

Alexander P.M. Guttenplan^*, Marijn Lemmens, Gilles Oudebrouckx, Daniel de Melo Pereira, Hoon Suk Rho, Zeinab Tahmasebi Birgani, Stefan Giselbrecht, Roman K. Truckenmüller, Ronald Thoelen, Pamela Habibovic

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

Abstract

We have developed a microfluidic chip which incorporates an electrical impedance sensor to detect the degradation of a biomimetic layer of calcium phosphate. This chip can be used to follow the resorption of model bone mineral by osteoclasts in real time without disturbing them, allowing the kinetics of this important biological process to be measured. The presence of multiple cell culture chambers allows parallel experiments with different conditions on one chip.

Original language	English
Title of host publication	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Publisher	Chemical and Biological Microsystems Society
Pages	1444-1445
Number of pages	2
ISBN (Electronic)	9781733419000
Publication status	Published - 1 Jan 2019
Event	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences - Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 Conference number: 23

Conference

Conference	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTAS 2019
Country/Territory	Switzerland
City	Basel
Period	27/10/19 → 31/10/19

Keywords

Bone
Cell Culture
Impedance
Sensors

Cite this

Guttenplan, A. P. M., Lemmens, M., Oudebrouckx, G., de Melo Pereira, D., Rho, H. S., Birgani, Z. T., Giselbrecht, S., Truckenmüller, R. K., Thoelen, R., & Habibovic, P. (2019). Real-time sensing of osteoclast activity by electrical impedance. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 1444-1445). Chemical and Biological Microsystems Society.

@inproceedings{04fc1e961e2a4c54aac8d8db2a0f8558,

title = "Real-time sensing of osteoclast activity by electrical impedance",

abstract = "We have developed a microfluidic chip which incorporates an electrical impedance sensor to detect the degradation of a biomimetic layer of calcium phosphate. This chip can be used to follow the resorption of model bone mineral by osteoclasts in real time without disturbing them, allowing the kinetics of this important biological process to be measured. The presence of multiple cell culture chambers allows parallel experiments with different conditions on one chip.",

keywords = "Bone, Cell Culture, Impedance, Sensors",

author = "Guttenplan, {Alexander P.M.} and Marijn Lemmens and Gilles Oudebrouckx and {de Melo Pereira}, Daniel and Rho, {Hoon Suk} and Birgani, {Zeinab Tahmasebi} and Stefan Giselbrecht and Truckenm{\"u}ller, {Roman K.} and Ronald Thoelen and Pamela Habibovic",

note = "Funding Information: This research has been made possible with the support of the Dutch Province of Limburg, the Interreg Vlaan-deren/Nederland BIOMAT-on-microfluidic-chip collaboration and the Netherlands Organisation for Scientific Research Vidi grant (15604). PH, RKT and SG gratefully acknowledge the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research (024.003.013). Funding Information: This research has been made possible with the support of the Dutch Province of Limburg, the Interreg Vlaanderen/Nederland BIOMAT-on-microfluidic-chip collaboration and the Netherlands Organisation for Scientific Research Vidi grant (15604). PH, RKT and SG gratefully acknowledge the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013). Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

month = jan,

day = "1",

language = "English",

pages = "1444--1445",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

publisher = "Chemical and Biological Microsystems Society",

address = "United States",

}

Guttenplan, APM, Lemmens, M, Oudebrouckx, G, de Melo Pereira, D, Rho, HS, Birgani, ZT , Giselbrecht, S , Truckenmüller, RK, Thoelen, R & Habibovic, P 2019, Real-time sensing of osteoclast activity by electrical impedance. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, pp. 1444-1445, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, Basel, Switzerland, 27/10/19.

Real-time sensing of osteoclast activity by electrical impedance. / Guttenplan, Alexander P.M.; Lemmens, Marijn; Oudebrouckx, Gilles et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. p. 1444-1445.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

TY - GEN

T1 - Real-time sensing of osteoclast activity by electrical impedance

AU - Guttenplan, Alexander P.M.

AU - Lemmens, Marijn

AU - Oudebrouckx, Gilles

AU - de Melo Pereira, Daniel

AU - Rho, Hoon Suk

AU - Birgani, Zeinab Tahmasebi

AU - Giselbrecht, Stefan

AU - Truckenmüller, Roman K.

AU - Thoelen, Ronald

AU - Habibovic, Pamela

N1 - Conference code: 23

PY - 2019/1/1

Y1 - 2019/1/1

N2 - We have developed a microfluidic chip which incorporates an electrical impedance sensor to detect the degradation of a biomimetic layer of calcium phosphate. This chip can be used to follow the resorption of model bone mineral by osteoclasts in real time without disturbing them, allowing the kinetics of this important biological process to be measured. The presence of multiple cell culture chambers allows parallel experiments with different conditions on one chip.

AB - We have developed a microfluidic chip which incorporates an electrical impedance sensor to detect the degradation of a biomimetic layer of calcium phosphate. This chip can be used to follow the resorption of model bone mineral by osteoclasts in real time without disturbing them, allowing the kinetics of this important biological process to be measured. The presence of multiple cell culture chambers allows parallel experiments with different conditions on one chip.

KW - Bone

KW - Cell Culture

KW - Impedance

KW - Sensors

UR - http://www.scopus.com/inward/record.url?scp=85094981010&partnerID=8YFLogxK

M3 - Conference article in proceeding

SP - 1444

EP - 1445

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

PB - Chemical and Biological Microsystems Society

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 27 October 2019 through 31 October 2019

ER -

Real-time sensing of osteoclast activity by electrical impedance

Abstract

Conference

Keywords

Other files and links

Fingerprint

Cite this