Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis

M. Matushek; M.J. Bonten; M. Hayden

doi:10.1128/JCM.34.10.2598-2600.1996

Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis

M. Matushek
, M.J. Bonten
, M. Hayden

NUTRIM - Institute of Nutrition and Translational Research in Metabolism

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.

Original language	English
Pages (from-to)	2598-600
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	34
Issue number	10
DOIs	https://doi.org/10.1128/JCM.34.10.2598-2600.1996
Publication status	Published - 1 Jan 1996

Access to Document

10.1128/JCM.34.10.2598-2600.1996

Cite this

@article{4c735419562b477dbd500b1cd08ce3ec,

title = "Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis",

abstract = "A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.",

author = "M. Matushek and M.J. Bonten and M. Hayden",

year = "1996",

month = jan,

day = "1",

doi = "10.1128/JCM.34.10.2598-2600.1996",

language = "English",

volume = "34",

pages = "2598--600",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "10",

}

TY - JOUR

T1 - Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis

AU - Matushek, M.

AU - Bonten, M.J.

AU - Hayden, M.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.

AB - A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.

U2 - 10.1128/JCM.34.10.2598-2600.1996

DO - 10.1128/JCM.34.10.2598-2600.1996

M3 - Article

SN - 0095-1137

VL - 34

SP - 2598

EP - 2600

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 10

ER -

Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis

Abstract

Access to Document

Fingerprint

Cite this