Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis.

M. Matushek, M.J. Bonten, M. Hayden

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Abstract

Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis.

Matushek MG, Bonten MJ, Hayden MK.

Department of Internal Medicine, Rush Medical College, Chicago, Illinois 60612, USA.

A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.
Original languageEnglish
Pages (from-to)240-240
JournalJournal of Clinical Microbiology
Volume15
Publication statusPublished - 1 Jan 1996

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