Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis

M. Matushek; M.J. Bonten; M. Hayden

doi:10.1128/JCM.34.10.2598-2600.1996

Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis

M. Matushek, M.J. Bonten, M. Hayden

NUTRIM School of Nutrition and Translational Research in Metabolism

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.

Original language	English
Pages (from-to)	2598-600
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	34
Issue number	10
DOIs	https://doi.org/10.1128/JCM.34.10.2598-2600.1996
Publication status	Published - 1 Jan 1996

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10.1128/JCM.34.10.2598-2600.1996

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Cite this

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title = "Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis",

abstract = "A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.",

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N2 - A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.

AB - A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.

U2 - 10.1128/JCM.34.10.2598-2600.1996

DO - 10.1128/JCM.34.10.2598-2600.1996

M3 - Article

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