Rapid genotyping of the OATP1B1 polymorphisms A388G and T521C with real-time PCR FRET assays

R.A. op den Buijsch, P.A.H.M. Wijnen, M.P. van Dieijen-Visser, J.E. de Vries, O. Bekers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The polymorphisms (OATP)1B1 A388G and T521C of the solute carrier organic anion-transporter family member 1B1 gene (SLCO1B1), previously known as OATP-C, have potential impacts on drug metabolism. In order to establish a fast and consistent assay for these polymorphisms, rapid speed polymerase chain reaction (PCR) fluorescence resonance energy transfer (FRET) assays on the LightCycler(R) were developed for both OATP1B1 polymorphisms. A locked nucleic acid (LNA) on the polymorphic location within the sensor probe was necessary to discriminate both alleles of the OATP1B1 T521C polymorphism. To confirm the reliability of both real-time PCR FRET assays, these new methods were validated by genotyping 120 samples using a PCR restriction fragment length polymorphism (RFLP) assay and an allele-specific PCR. The results of the real-time PCR FRET assays were completely in line with conventional PCR methods, indicating that the real-time PCR FRET assays are appropriate for clinical settings.
Original languageEnglish
Pages (from-to)393-397
JournalPharmacogenomics
Volume6
Issue number4
DOIs
Publication statusPublished - 1 Jan 2005

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