Quantitative T-2 Mapping of the Mouse Heart by Segmented MLEV Phase-Cycled T-2 Preparation

Bram F. Coolen, Frank F. J. Simonis, Tessa Geelen, Rik P. M. Moonen, Fatih Arslan, Leonie E. M. Paulis, Klaas Nicolay, Gustav J. Strijkers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Web of Science)

Abstract

A high-quality, reproducible, multi-slice T2-mapping protocol for the mouse heart is presented.A T2-prepared sequence with composite 90? and 180? radiofrequency pulses in a segmented MLEV phase cycling scheme was developed. The T2-mapping protocol was optimized using simulations and evaluated with phantoms.Repeatability for determination of myocardial T2 values was assessed in vivo in n?=?5 healthy mice on 2 different days. The average baseline T2 of the left ventricular myocardium was 22.5?1.7 ms. The repeatability coefficient for R2?=?1/T2 for measurements at different days was ?R2?=?6.3 s(?1). Subsequently, T2 mapping was applied in comparison to late-gadolinium-enhancement (LGE) imaging, to assess 1-day-old ischemia/reperfusion (IR) myocardial injury in n?=?8 mice. T2 in the infarcts was significantly higher than in remote tissue, whereas remote tissue was not significantly different from baseline. Infarct sizes based on T2 versus LGE showed strong correlation. To assess the time-course of T2 changes in the infarcts, T2 mapping was performed at day 1, 3, and 7 after IR injury in a separate group of mice (n?=?16). T2 was highest at day 3, in agreement with the expected time course of edema formation and resolution after myocardial infarction.T2 prepared imaging provides high quality reproducible T2 maps of healthy and diseased mouse myocardium.2013
Original languageEnglish
Pages (from-to)409-417
JournalMagnetic Resonance in Medicine
Volume72
Issue number2
DOIs
Publication statusPublished - Aug 2014

Keywords

  • cardiac MRI
  • T-2 mapping
  • quantitative imaging
  • myocardial infarction
  • mouse models

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