Purified exosome product enhances chondrocyte survival and regeneration by modulating inflammation and promoting chondrogenesis

Guidong Shi; Zeling Long; Rodolfo E De la Vega; Atta Behfar; Steven L Moran; Christopher Evans; Chunfeng Zhao

doi:10.2217/rme-2022-0132

Purified exosome product enhances chondrocyte survival and regeneration by modulating inflammation and promoting chondrogenesis

Guidong Shi
, Zeling Long
, Rodolfo E De la Vega
, Atta Behfar
, Steven L Moran
, Christopher Evans
, Chunfeng Zhao^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Aim: This study was to detect the effects of purified exosome product (PEP) on C28/I2 cells and chondrocytes derived from osteoarthritis patients. Materials & methods: Cell viability and apoptosis assays were used to detect the effect of PEP on cells. qRT-PCR and cell fluorescence assays were used to investigate the potential mechanism of PEP on cell chondrogenesis. Results: PEP was internalized by cells at a fast rate and enhanced cellular proliferation and migration while attenuating apoptosis. These findings reflect the fact that PEP can increase the expression of PCNA and reduce the expression of CASP3/7/9 and BAX. Conclusion: This study suggests an innovative strategy for chondrogenesis that could be applied to osteoarthritis repair in the future.

Original language	English
Pages (from-to)	55-71
Number of pages	17
Journal	Regenerative Medicine
Volume	18
Issue number	1
Early online date	18 Oct 2022
DOIs	https://doi.org/10.2217/rme-2022-0132
Publication status	Published - Jan 2023

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https://www.ncbi.nlm.nih.gov/pmc/articles/9732920Licence: Other

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title = "Purified exosome product enhances chondrocyte survival and regeneration by modulating inflammation and promoting chondrogenesis",

abstract = "Aim: This study was to detect the effects of purified exosome product (PEP) on C28/I2 cells and chondrocytes derived from osteoarthritis patients. Materials \& methods: Cell viability and apoptosis assays were used to detect the effect of PEP on cells. qRT-PCR and cell fluorescence assays were used to investigate the potential mechanism of PEP on cell chondrogenesis. Results: PEP was internalized by cells at a fast rate and enhanced cellular proliferation and migration while attenuating apoptosis. These findings reflect the fact that PEP can increase the expression of PCNA and reduce the expression of CASP3/7/9 and BAX. Conclusion: This study suggests an innovative strategy for chondrogenesis that could be applied to osteoarthritis repair in the future.",

author = "Guidong Shi and Zeling Long and \{De la Vega\}, \{Rodolfo E\} and Atta Behfar and Moran, \{Steven L\} and Christopher Evans and Chunfeng Zhao",

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doi = "10.2217/rme-2022-0132",

language = "English",

volume = "18",

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T1 - Purified exosome product enhances chondrocyte survival and regeneration by modulating inflammation and promoting chondrogenesis

AU - Shi, Guidong

AU - Long, Zeling

AU - De la Vega, Rodolfo E

AU - Behfar, Atta

AU - Moran, Steven L

AU - Evans, Christopher

AU - Zhao, Chunfeng

PY - 2023/1

Y1 - 2023/1

N2 - Aim: This study was to detect the effects of purified exosome product (PEP) on C28/I2 cells and chondrocytes derived from osteoarthritis patients. Materials & methods: Cell viability and apoptosis assays were used to detect the effect of PEP on cells. qRT-PCR and cell fluorescence assays were used to investigate the potential mechanism of PEP on cell chondrogenesis. Results: PEP was internalized by cells at a fast rate and enhanced cellular proliferation and migration while attenuating apoptosis. These findings reflect the fact that PEP can increase the expression of PCNA and reduce the expression of CASP3/7/9 and BAX. Conclusion: This study suggests an innovative strategy for chondrogenesis that could be applied to osteoarthritis repair in the future.

AB - Aim: This study was to detect the effects of purified exosome product (PEP) on C28/I2 cells and chondrocytes derived from osteoarthritis patients. Materials & methods: Cell viability and apoptosis assays were used to detect the effect of PEP on cells. qRT-PCR and cell fluorescence assays were used to investigate the potential mechanism of PEP on cell chondrogenesis. Results: PEP was internalized by cells at a fast rate and enhanced cellular proliferation and migration while attenuating apoptosis. These findings reflect the fact that PEP can increase the expression of PCNA and reduce the expression of CASP3/7/9 and BAX. Conclusion: This study suggests an innovative strategy for chondrogenesis that could be applied to osteoarthritis repair in the future.

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DO - 10.2217/rme-2022-0132

M3 - Article

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SN - 1746-0751

VL - 18

SP - 55

EP - 71

JO - Regenerative Medicine

JF - Regenerative Medicine

IS - 1

ER -

Purified exosome product enhances chondrocyte survival and regeneration by modulating inflammation and promoting chondrogenesis

Abstract

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