Purification of blood coagulation factors II, VII, IX and X from bovine citrated plasma

P. Reekers, H.C. Hemker

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    Abstract

    The bovine coagulation factors II, VII, IX and X were separated from other plasma proteins, and each factor was obtained in a largely pure state by various chromatographic procedures in the presence of diisopropylfluorophosphate (DFP). Factor X was separated from factors II, VII, and IX by DEAE Sephadex chromatography, followed by G-100 filtration and hydroxylapatite chromatography. Separation of factors II and IX from factor VII was achieved by chromatography on DEAE cellulose, followed by G-100 gel filtration and hydroxylapatite chromatography. Factors II and IX were separated by preparative polyacrylamide electrophoresis. After DEAE cellulose chromatography, factor VII was purified by gradient elution from hydroxylapatite.
    Original languageEnglish
    Pages (from-to)2-22
    Number of pages21
    JournalPathophysiology of Haemostasis and Thrombosis
    Volume1
    Issue number1
    DOIs
    Publication statusPublished - 1972

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