Protocol for differentiation of bovine adipogenic progenitor cells embedded in alginate sheets

Joseph J. Caponi, Jane Shmushkis, Hanna Geissler, Mark J. Post, Laura Jackisch, Eva M. Mall*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Here, we present a cost-effective protocol to differentiate bovine fibro-adipogenic progenitors in a thin hydrogel sheet adherent to 96-well plates. We describe steps for the embedding and culturing of cells in alginate sheets, culture maintenance, and analysis. Compared to alternative three-dimensional (3D) models such as hydrogel-based microfibers, this approach simplifies automation while retaining efficient maturation of adipocytes. Embedded cells are still subjected to a 3D environment, but the sheets can be handled and analyzed like two-dimensional cultures.
Original languageEnglish
Article number102143
Number of pages18
JournalSTAR protocols
Volume4
Issue number2
DOIs
Publication statusPublished - 16 Jun 2023

Keywords

  • Cell Biology
  • Cell Culture
  • Cell Differentiation
  • Cell Isolation
  • Cell-based Assays
  • High-throughput Screening
  • Microscopy
  • Stem Cells

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