Prothrombotic State in Asthma Is Related to Increased Levels of Inflammatory Cytokines, IL-6 and TNF alpha, in Peripheral Blood

Stanislawa Bazan-Socha, Lucyna Mastalerz, Agnieszka Cybulska, Lech Zareba, Romy Kremers, Michal Zabczyk, Grazyna Pulka, Teresa Iwaniec, Coenraad Hemker, Anetta Undas*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Web of Science)

Abstract

Recently, we have reported that asthma is associated with enhanced plasma thrombin formation and impaired fibrinolysis. The mechanisms underlying the prothrombotic state in this disease are unknown. Our aim was to investigate whether prothrombotic alterations in asthmatics are associated with inflammation. We studied 164 adult, white, stable asthmatics and 72 controls matched for age, sex, body mass index (BMI), and smoking. Plasma tumor necrosis factor alpha (TNF alpha), interleukin (IL)-6, and serum periostin were evaluated using ELISAs, and their associations with thrombin generation, fibrinolytic capacity, expressed as clot lysis time (CLT), and platelet markers were later analyzed. Asthma was characterized by 62% higher plasma IL-6 and 35% higher TNF alpha (both, p <0.0001). Inflammatory cytokines were higher in sporadic and persistent asthmatics compared to controls, also after adjustment for potential confounders. IL-6 was inversely related to the forced expiratory volume in 1 s/vital capacity (FEV1/VC) spirometry index after correction for age, sex, and BMI. IL-6 and TNF alpha were associated with C-reactive protein in asthmatics (beta = 0.6 [95% CI, 0.54-0.67] and beta = 0.33 [95% CI, 0.25-0.41], respectively) and controls (beta = 0.43 [95% CI, 0.29-0.57] and beta = 0.33 [95% CI, 0.18-0.48], respectively). In asthma, IL-6 and TNF alpha positively correlated with the endogenous thrombin potential (beta = 0.35 [95% CI, 0.28-0.42] and beta = 0.15 [95% CI, 0.07-0.23], respectively) but not with CLT or platelet markers. However, TNF alpha predicted CLT in a multiple linear regression model. Periostin was not associated with any hemostatic parameters. Enhanced thrombin generation is driven in asthma by a systemic inflammatory state mediated by IL-6 and to a lesser extent TNF alpha, however, not periostin. TNF alpha might contribute to impaired fibrinolysis.

Original languageEnglish
Pages (from-to)1225-1235
Number of pages11
JournalInflammation
Volume40
Issue number4
DOIs
Publication statusPublished - Aug 2017

Keywords

  • Asthma
  • Thrombin generation
  • Fibrinolysis
  • IL-6
  • TNF alpha
  • Periostin
  • TUMOR-NECROSIS-FACTOR
  • CORONARY-HEART-DISEASE
  • ADULT-ONSET ASTHMA
  • PLASMINOGEN-ACTIVATOR
  • TISSUE FACTOR
  • SYSTEMIC INFLAMMATION
  • INTERLEUKIN-6
  • RISK
  • COAGULATION
  • PERIOSTIN

Cite this