TY - JOUR
T1 - Proteomics analysis of zebrafish larvae exposed to 3,4-dichloroaniline using the fish embryo acute toxicity test
AU - Vieira, Leonardo R.
AU - Hissa, Denise C.
AU - de Souza, Terezinha Maria
AU - Sa, Chayenne A.
AU - Evaristo, Joseph A. M.
AU - Nogueira, Fabio C. S.
AU - Carvalho, Ana F. U.
AU - Farias, Davi F.
N1 - Funding Information:
We thank to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, for supporting this research with grants (Grant number 461182/2014‐9) and scholarships.
Publisher Copyright:
© 2020 Wiley Periodicals, Inc.
PY - 2020/8
Y1 - 2020/8
N2 - The zebrafish (Danio rerio) is a small teleost fish that is becoming increasingly popular in laboratories worldwide and several attributes have also placed the zebrafish under the spotlight of (eco)toxicological studies. Since the 1990s, international organizations such as ISO and OECD have published guidelines for the use of zebrafish in ecotoxicological assessment of environmental toxicants such as the Fish Embryo Acute Toxicity (FET) test, OECD n degrees 236 guideline. This protocol uses 3,4-dichloroaniline (DCA), an aniline pesticide whose toxicity to fish species at early life stages is well known, as a positive control. Despite its use, little is known about its molecular mechanisms, especially in the context of the FET test. Therefore, this study aimed to investigate such changes in zebrafish larvae exposed to DCA (4 mg/L) for 96 hours using gel-free proteomics. Twenty-four proteins detected in both groups were identified as significantly affected by DCA exposure, and, when considering group-specific entities, 48 proteins were exclusive to DCA (group-specific proteins) while 248 were only detected in the control group. Proteins modulated by DCA treatment were found to be involved in metabolic processes, especially lipids and hormone metabolism (eg, Apoa1 and Apoa1b and vitelogenins), as well as proteins important for developmental processes and organogenesis (eg, Myhc4, Acta2, Sncb, and Marcksb). The results presented here may therefore provide a better understanding of the relationships between molecular changes and phenotype in zebrafish larvae treated with DCA, the reference compound of the FET test.
AB - The zebrafish (Danio rerio) is a small teleost fish that is becoming increasingly popular in laboratories worldwide and several attributes have also placed the zebrafish under the spotlight of (eco)toxicological studies. Since the 1990s, international organizations such as ISO and OECD have published guidelines for the use of zebrafish in ecotoxicological assessment of environmental toxicants such as the Fish Embryo Acute Toxicity (FET) test, OECD n degrees 236 guideline. This protocol uses 3,4-dichloroaniline (DCA), an aniline pesticide whose toxicity to fish species at early life stages is well known, as a positive control. Despite its use, little is known about its molecular mechanisms, especially in the context of the FET test. Therefore, this study aimed to investigate such changes in zebrafish larvae exposed to DCA (4 mg/L) for 96 hours using gel-free proteomics. Twenty-four proteins detected in both groups were identified as significantly affected by DCA exposure, and, when considering group-specific entities, 48 proteins were exclusive to DCA (group-specific proteins) while 248 were only detected in the control group. Proteins modulated by DCA treatment were found to be involved in metabolic processes, especially lipids and hormone metabolism (eg, Apoa1 and Apoa1b and vitelogenins), as well as proteins important for developmental processes and organogenesis (eg, Myhc4, Acta2, Sncb, and Marcksb). The results presented here may therefore provide a better understanding of the relationships between molecular changes and phenotype in zebrafish larvae treated with DCA, the reference compound of the FET test.
KW - chemical contaminants
KW - endocrine disruptors
KW - OECD FET test guideline
KW - proteomics
KW - reference compound
KW - DEVELOPMENTAL TOXICITY
KW - RERIO
KW - METABOLISM
KW - EXPRESSION
KW - STRESS
KW - MODEL
U2 - 10.1002/tox.22921
DO - 10.1002/tox.22921
M3 - Article
C2 - 32170993
SN - 1520-4081
VL - 35
SP - 849
EP - 860
JO - Environmental Toxicology
JF - Environmental Toxicology
IS - 8
ER -