Proteomic Correlates and Prognostic Significance of Kidney Injury in Heart Failure With Preserved Ejection Fraction

Oday Salman, Lei Zhao, Jordana B. Cohen, Marie Joe Dib, Joe David Azzo, Sushrima Gan, A. Mark Richards, Bianca Pourmussa, Rob Doughty, Ali Javaheri, Douglas L. Mann, Ernst Rietzschel, Manyun Zhao, Zhaoqing Wang, Christina Ebert, Vanessa van Empel, Karl Kammerhoff, Joseph Maranville, Joseph Gogain, Jaclyn DennisPeter H. Schafer, Dietmar Seiffert, David A. Gordon, Francisco Ramirez-Valle, Thomas P. Cappola, Julio A. Chirinos*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

BACKGROUND: Kidney disease is common in heart failure with preserved ejection fraction (HFpEF). However, the biologic correlates and prognostic significance of kidney injury (KI), in HFpEF, beyond the estimated glomerular filtration rate (eGFR), are unclear. METHODS AND RESULTS: Using baseline plasma samples from the TOPCAT (Treatment of Preserved Cardiac Function Heart Failure With an Aldosterone Antagonist) trial, we measured the following KI biomarkers: cystatin-C, fatty acid-binding protein-3, Beta-2 microglobulin, neutrophil gelatinase-associated lipocalin, and kidney-injury molecule-1. Factor analysis was used to extract the common variability underlying these biomarkers. We assessed the relationship between the KI-factor score and the risk of death or HF-related hospital admission in models adjusted for the Meta-Analysis Global Group in Chronic Heart Failure risk score and eGFR. We also assessed the relationship between the KI factor score and ~5000 plasma proteins, followed by pathway analysis. We validated our findings among HFpEF participants in the Penn Heart Failure Study. KI was associated with the risk of death or HF-related hospital admission independent of the Meta-Analysis Global Group in Chronic Heart Failure risk score and eGFR. Both the risk score and eGFR were no longer associated with death or HF-related hospital admission after adjusting for the KI factor score. KI was predominantly associated with proteins and biologic pathways related to complement activation, inflammation, fibrosis, and cholesterol homeostasis. KI was associated with 140 proteins, which reproduced across cohorts. Findings regarding biologic associations and the prognostic significance of KI were also reproduced in the validation cohort. CONCLUSIONS: KI is associated with adverse outcomes in HFpEF independent of baseline eGFR. Patients with HFpEF and KI exhibit a plasma proteomic signature indicative of complement activation, inflammation, fibrosis, and impaired cholesterol homeostasis.
Original languageEnglish
Article numbere033660
JournalJournal of the American Heart Association
Volume13
Issue number17
DOIs
Publication statusPublished - 3 Sept 2024

Keywords

  • cardiorenal interactions
  • chronic kidney disease
  • HFpEF
  • kidney injury
  • outcomes
  • proteomics

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