Proteomic analysis of log to stationary growth phase Lactobacillus plantarum cells and a 2-DE database

D.P. Cohen, J.W. Renes, F.G. Bouwman, E.G. Zoetendaal, E.C.M. Mariman, W.M. de Vos, E.E. Vaughan

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Abstract

Lactobacillus plantarum is part of the natural microbiota of many food fermentations as well as the human gastro-intestinal tract. The cytosolic fraction of the proteome of L. plantarum WCFS1, whose genome has been sequenced, was studied. 2-DE was used to investigate the proteins from the cytosolic fraction isolated from mid- and late-log, early- and late-stationary phase cells to generate reference maps of different growth conditions offering more knowledge of the metabolic behavior of this bacterium. From this fraction, a total of 200 protein spots were identified by MALDI-MS and a proteome production map was constructed to facilitate further studies such as detection of suitable biomarkers for specific growth conditions. More than half (57%) of the identified proteins were predicted to be involved in metabolic pathways of the bacterium. The protein profile changed during the growth of the bacteria such that 29% of the identified proteins involved in anabolic pathways were at least twofold up-regulated throughout the mid- and late-exponential and early-stationary phases. In the late-stationary phase, six proteins involved in stress or with a potential role for survival during starvation were up-regulated significantly
Original languageEnglish
Pages (from-to)6485-6493
JournalProteomics
Volume6
Issue number24
DOIs
Publication statusPublished - 1 Jan 2006

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