Protein leverage effects of beef protein on energy intake in humans

E.A. Martens, S.Y. Tan, M.V. Dunlop, R.D. Mattes, M.S. Westerterp-Plantenga

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

BACKGROUND: The protein leverage hypothesis requires specific evidence that protein intake is regulated more strongly than energy intake. OBJECTIVE: The objective was to determine ad libitum energy intake, body weight changes, appetite profile, and nitrogen balance in response to 3 diets with different protein-to-carbohydrate + fat ratios over 12 consecutive days, with beef as a source of protein. DESIGN: A 3-arm, 12-d randomized crossover study was performed in 30 men and 28 women [mean +/- SD age: 33 +/- 16 y; body mass index (in kg/m2): 24.4 +/- 4.0] with the use of diets containing 5%, 15%, and 30% of energy (En%) from protein, predominantly from beef. RESULTS: Energy intake was significantly lower in the 30En%-protein condition (8.73 +/- 1.93 MJ/d) than in the 5En%-protein (9.48 +/- 1.67 MJ/d) and 15En%-protein (9.30 +/- 1.62 MJ/d) conditions (P = 0.001), stemming largely from lower energy intake during meals (P = 0.001). Hunger (P = 0.001) and desire to eat (P = 0.001) ratings were higher and fullness ratings were lower (P = 0.001) in the 5En%-protein condition than in the 15En%-protein and 30En%-protein conditions. Nitrogen excretion was lower in the 5En%-protein condition (4.7 +/- 1.5 g/24 h; P = 0.001) and was higher in the 30En%-protein condition (15.3 +/- 8.7 g/24 h; P = 0.001) compared with the 15En%-protein condition (10.0 +/- 5.2 g/24 h). Nitrogen balance was maintained in the 5En%-protein condition and was positive in the 15En%- and 30En%-protein conditions (P = 0.001). CONCLUSIONS: Complete protein leverage did not occur because subjects did not consume to a common protein amount at the expense of energy balance. Individuals did underconsume relative to energy requirements from high-protein diets. The lack of support for protein leverage effects on a low-protein diet may stem from the fact that protein intake was sufficient to maintain nitrogen balance over the 12-d trial. This trial was registered at clinicaltrials.gov as NCT01646749.
Original languageEnglish
Pages (from-to)1397-1406
Number of pages10
JournalAmerican Journal of Clinical Nutrition
Volume99
Issue number6
DOIs
Publication statusPublished - Jun 2014

Keywords

  • AMINO-ACID DEFICIENCY
  • DIETARY-PROTEIN
  • WEIGHT-LOSS
  • BODY-WEIGHT
  • FOOD-INTAKE
  • PHYSICAL-ACTIVITY
  • FEEDING-BEHAVIOR
  • FAT
  • APPETITE
  • SATIETY

Cite this

Martens, E. A., Tan, S. Y., Dunlop, M. V., Mattes, R. D., & Westerterp-Plantenga, M. S. (2014). Protein leverage effects of beef protein on energy intake in humans. American Journal of Clinical Nutrition, 99(6), 1397-1406. https://doi.org/10.3945/ajcn.113.078774