Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes

C. S. Shaw, S. O. Shepherd, A.J. Wagenmakers, D. Hansen, P. Dendale, L.J. van Loon

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The aim of the present study was to investigate changes in intramuscular triglyceride (IMTG) content and perilipin 2 expression in skeletal muscle tissue following 6 mo of endurance-type exercise training in type 2 diabetes patients. Ten obese male type 2 diabetes patients (age 62 +/- 1 yr, body mass index BMI 31 +/- 1 kg/m(2)) completed three exercise sessions/week consisting of 40 min of continuous endurance-type exercise at 75% V(O(2) peak) for a period of 6 mo. Muscle biopsies collected at baseline and after 2 and 6 mo of intervention were analyzed for IMTG content and perilipin 2 expression using fiber type-specific immunofluorescence microscopy. Endurance-type exercise training reduced trunk body fat by 6 +/- 2% and increased whole body oxygen uptake capacity by 13 +/- 7% (P < 0.05). IMTG content increased twofold in response to the 6 mo of exercise training in both type I and type II muscle fibers (P < 0.05). A threefold increase in perilipin 2 expression was observed from baseline to 2 and 6 mo of intervention in the type I muscle fibers only (1.1 +/- 0.3, 3.4 +/- 0.6, and 3.6 +/- 0.6% of fibers stained, respectively, P < 0.05). Exercise training induced a 1.6-fold increase in mitochondrial content after 6 mo of training in both type I and type II muscle fibers (P < 0.05). In conclusion, this is the first study to report that prolonged endurance-type exercise training increases the expression of perilipin 2 alongside increases in IMTG content in a type I muscle fiber-type specific manner in type 2 diabetes patients.
Original languageEnglish
Pages (from-to)E1158-65
JournalAmerican Journal of Physiology : Endocrinology and Metabolism
Volume303
Issue number9
DOIs
Publication statusPublished - 1 Jan 2012

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