The aim of the present study was to investigate changes in intramuscular triglyceride (IMTG) content and perilipin 2 expression in skeletal muscle tissue following 6 mo of endurance-type exercise training in type 2 diabetes patients. Ten obese male type 2 diabetes patients (age 62 +/- 1 yr, body mass index BMI 31 +/- 1 kg/m(2)) completed three exercise sessions/week consisting of 40 min of continuous endurance-type exercise at 75% V(O(2) peak) for a period of 6 mo. Muscle biopsies collected at baseline and after 2 and 6 mo of intervention were analyzed for IMTG content and perilipin 2 expression using fiber type-specific immunofluorescence microscopy. Endurance-type exercise training reduced trunk body fat by 6 +/- 2% and increased whole body oxygen uptake capacity by 13 +/- 7% (P < 0.05). IMTG content increased twofold in response to the 6 mo of exercise training in both type I and type II muscle fibers (P < 0.05). A threefold increase in perilipin 2 expression was observed from baseline to 2 and 6 mo of intervention in the type I muscle fibers only (1.1 +/- 0.3, 3.4 +/- 0.6, and 3.6 +/- 0.6% of fibers stained, respectively, P < 0.05). Exercise training induced a 1.6-fold increase in mitochondrial content after 6 mo of training in both type I and type II muscle fibers (P < 0.05). In conclusion, this is the first study to report that prolonged endurance-type exercise training increases the expression of perilipin 2 alongside increases in IMTG content in a type I muscle fiber-type specific manner in type 2 diabetes patients.
|Journal||American Journal of Physiology : Endocrinology and Metabolism|
|Publication status||Published - 1 Jan 2012|
Shaw, C. S., Shepherd, S. O., Wagenmakers, A. J., Hansen, D., Dendale, P., & van Loon, L. J. (2012). Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes. American Journal of Physiology : Endocrinology and Metabolism, 303(9), E1158-65. https://doi.org/10.1152/ajpendo.00272.2012