Presleep protein ingestion does not compromise the muscle protein synthetic response to protein ingested the following morning

Benjamin T. Wall, Nick Burd, Rinske Franssen, Stefan H. M. Gorissen, Tim Snijders, Joannes Senden, Annemie P. Gijsen, Lucas van Loon*

*Corresponding author for this work

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Protein ingestion before sleep augments postexercise muscle protein synthesis during overnight recovery. It is unknown whether postexercise and presleep protein consumption modulates postprandial protein handling and myofibrillar protein synthetic responses the following morning. Sixteen healthy young (24 +/- 1 yr) men performed unilateral resistance-type exercise (contralateral leg acting as a resting control) at 2000. Participants ingested 20 g of protein immediately after exercise plus 60 g of protein presleep (PRO group; n = 8) or equivalent boluses of carbohydrate (CON; n = 8). The subsequent morning participants received primed, continuous infusions of L-[ring-H-2(5)] phenylalanine and L-[1-C-13] leucine combined with ingestion of 20 g intrinsically L-[1-C-13] phenylalanineand L-[1-C-13] leucine-labeled protein to assess postprandial protein handling and myofibrillar protein synthesis in the rested and exercised leg in CON and PRO. Exercise increased postabsorptive myofibrillar protein synthesis rates the subsequent day (P <0.001), with no differences between CON and PRO. Protein ingested in the morning increased myofibrillar protein synthesis in both the exercised and rested leg (P <0.01), with no differences between treatments. Myofibrillar protein bound L-[1-C-13] phenylalanine enrichments were greater in the exercised (0.016 +/- 0.002 and 0.015 +/- 0.002 MPE in CON and PRO, respectively) vs. rested (0.010 +/- 0.002 and 0.009 +/- 0.002 MPE in CON and PRO, respectively) leg (P
Original languageEnglish
Pages (from-to)E964-E973
JournalAmerican Journal of Physiology : Endocrinology and Metabolism
Issue number6
Publication statusPublished - 1 Dec 2016


  • skeletal muscle
  • amino acids
  • protein synthesis
  • resistance-type exercise

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