Prenatal exposure to flavonoids: implication for cancer risk

K. Vanhees, L. de Bock, R.W.L. Godschalk, F.J. van Schooten, S.B. Khosrovani

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Flavonoids are potent antioxidants, freely available as high-dose dietary supplements. However, they can induce DNA double-strand breaks (DSB) and rearrangements in the mixed-lineage leukemia (MLL) gene, which are frequently observed in childhood leukemia. We hypothesize that a deficient DSB repair, as a result of an Atm mutation, may reinforce the clastogenic effect of dietary flavonoids and increase the frequency of Mll rearrangements. Therefore, we examined the effects of in vitro and transplacental exposure to high, but biological amounts of flavonoids in mice with different genetic capacities for DSB repair (homozygous/heterozygous knock-in for human Atm mutation [Atm-delta SRI] vs. wild type [wt]). In vitro exposure to genistein/quercetin induced higher numbers of Mll rearrangements in bone marrow cells of Atm-delta SRI mutant mice compared with wt mice. Subsequently, heterozygous Atm-delta SRI mice were placed on either a flavonoid-poor or a genistein-enriched (270 mg/kg) or quercetin-enriched (302 mg/kg) feed throughout pregnancy. Prenatal exposure to flavonoids associated with higher frequencies of Mll rearrangements and a slight increase in the incidence of malignancies in DNA repair-deficient mice. These data suggest that prenatal exposure to both genistein and quercetin supplements could increase the risk on Mll rearrangements especially in the presence of compromised DNA repair.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalToxicological Sciences
Volume120
Issue number1
DOIs
Publication statusPublished - Mar 2011

Keywords

  • genistein
  • quercetin
  • Mll translocations
  • infant leukemia
  • in utero
  • Atm gene
  • DNA TOPOISOMERASE-II
  • STRAND BREAK REPAIR
  • INFANT LEUKEMIA
  • DIETARY FLAVONOIDS
  • MLL GENE
  • IN-VIVO
  • ATAXIA-TELANGIECTASIA
  • ATM MUTATIONS
  • QUERCETIN
  • ETOPOSIDE

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