Preferential uptake of dietary Fatty acids in adipose tissue and muscle in the postprandial period

A.S. Bickerton, R. Roberts, B.A. Fielding, L. Hodson, E.E. Blaak, A.J. Wagenmakers, M. Gilbert, F. Karpe, K.N. Frayn

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Despite consistent evidence that abnormalities of fatty acid delivery and storage underlie the metabolic defects of insulin resistance, physiological pathways by which fat is stored in adipose tissue and skeletal muscle are not clear. We used a combination of stable isotope labeling and arteriovenous difference measurements to elucidate pathways of postprandial fat deposition in adipose tissue and skeletal muscle in healthy humans. A test meal containing [U-(13)C]palmitate was combined with intravenous infusion of [(2)H(2)]palmitate to label plasma fatty acids and VLDL-triglyceride. Both dietary (chylomicron) and VLDL-triglyceride were cleared across adipose tissue and muscle, though with greater fractional extraction of the chylomicron-triglyceride. In adipose tissue there was significant uptake of plasma nonesterified fatty acids (NEFAs) in the postprandial but not the fasting state. However, this was minor in comparison with chylomicron-triglyceride fatty acids. We modeled the fate of fatty acids released by lipoprotein lipase (LPL). There was clear preferential uptake of these fatty acids compared with plasma NEFAs. In muscle, there was unexpected evidence for release of LPL-derived fatty acids into the plasma. With this integrative physiological approach, we have revealed hidden complexities in pathways of fatty acid uptake in adipose tissue and skeletal muscle. AD - Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, Old Road, Headington, Oxford, OX3 7LJ, U.K. keith.frayn@oxlip.ox.ac.uk.
Original languageEnglish
Pages (from-to)168-176
JournalDiabetes
Volume56
Issue number1
DOIs
Publication statusPublished - 1 Jan 2007

Cite this

Bickerton, A. S., Roberts, R., Fielding, B. A., Hodson, L., Blaak, E. E., Wagenmakers, A. J., ... Frayn, K. N. (2007). Preferential uptake of dietary Fatty acids in adipose tissue and muscle in the postprandial period. Diabetes, 56(1), 168-176. https://doi.org/10.2337/db06-0822
Bickerton, A.S. ; Roberts, R. ; Fielding, B.A. ; Hodson, L. ; Blaak, E.E. ; Wagenmakers, A.J. ; Gilbert, M. ; Karpe, F. ; Frayn, K.N. / Preferential uptake of dietary Fatty acids in adipose tissue and muscle in the postprandial period. In: Diabetes. 2007 ; Vol. 56, No. 1. pp. 168-176.
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Bickerton, AS, Roberts, R, Fielding, BA, Hodson, L, Blaak, EE, Wagenmakers, AJ, Gilbert, M, Karpe, F & Frayn, KN 2007, 'Preferential uptake of dietary Fatty acids in adipose tissue and muscle in the postprandial period', Diabetes, vol. 56, no. 1, pp. 168-176. https://doi.org/10.2337/db06-0822

Preferential uptake of dietary Fatty acids in adipose tissue and muscle in the postprandial period. / Bickerton, A.S.; Roberts, R.; Fielding, B.A.; Hodson, L.; Blaak, E.E.; Wagenmakers, A.J.; Gilbert, M.; Karpe, F.; Frayn, K.N.

In: Diabetes, Vol. 56, No. 1, 01.01.2007, p. 168-176.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Preferential uptake of dietary Fatty acids in adipose tissue and muscle in the postprandial period

AU - Bickerton, A.S.

AU - Roberts, R.

AU - Fielding, B.A.

AU - Hodson, L.

AU - Blaak, E.E.

AU - Wagenmakers, A.J.

AU - Gilbert, M.

AU - Karpe, F.

AU - Frayn, K.N.

PY - 2007/1/1

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N2 - Despite consistent evidence that abnormalities of fatty acid delivery and storage underlie the metabolic defects of insulin resistance, physiological pathways by which fat is stored in adipose tissue and skeletal muscle are not clear. We used a combination of stable isotope labeling and arteriovenous difference measurements to elucidate pathways of postprandial fat deposition in adipose tissue and skeletal muscle in healthy humans. A test meal containing [U-(13)C]palmitate was combined with intravenous infusion of [(2)H(2)]palmitate to label plasma fatty acids and VLDL-triglyceride. Both dietary (chylomicron) and VLDL-triglyceride were cleared across adipose tissue and muscle, though with greater fractional extraction of the chylomicron-triglyceride. In adipose tissue there was significant uptake of plasma nonesterified fatty acids (NEFAs) in the postprandial but not the fasting state. However, this was minor in comparison with chylomicron-triglyceride fatty acids. We modeled the fate of fatty acids released by lipoprotein lipase (LPL). There was clear preferential uptake of these fatty acids compared with plasma NEFAs. In muscle, there was unexpected evidence for release of LPL-derived fatty acids into the plasma. With this integrative physiological approach, we have revealed hidden complexities in pathways of fatty acid uptake in adipose tissue and skeletal muscle. AD - Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, Old Road, Headington, Oxford, OX3 7LJ, U.K. keith.frayn@oxlip.ox.ac.uk.

AB - Despite consistent evidence that abnormalities of fatty acid delivery and storage underlie the metabolic defects of insulin resistance, physiological pathways by which fat is stored in adipose tissue and skeletal muscle are not clear. We used a combination of stable isotope labeling and arteriovenous difference measurements to elucidate pathways of postprandial fat deposition in adipose tissue and skeletal muscle in healthy humans. A test meal containing [U-(13)C]palmitate was combined with intravenous infusion of [(2)H(2)]palmitate to label plasma fatty acids and VLDL-triglyceride. Both dietary (chylomicron) and VLDL-triglyceride were cleared across adipose tissue and muscle, though with greater fractional extraction of the chylomicron-triglyceride. In adipose tissue there was significant uptake of plasma nonesterified fatty acids (NEFAs) in the postprandial but not the fasting state. However, this was minor in comparison with chylomicron-triglyceride fatty acids. We modeled the fate of fatty acids released by lipoprotein lipase (LPL). There was clear preferential uptake of these fatty acids compared with plasma NEFAs. In muscle, there was unexpected evidence for release of LPL-derived fatty acids into the plasma. With this integrative physiological approach, we have revealed hidden complexities in pathways of fatty acid uptake in adipose tissue and skeletal muscle. AD - Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, Old Road, Headington, Oxford, OX3 7LJ, U.K. keith.frayn@oxlip.ox.ac.uk.

U2 - 10.2337/db06-0822

DO - 10.2337/db06-0822

M3 - Article

VL - 56

SP - 168

EP - 176

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 1

ER -

Bickerton AS, Roberts R, Fielding BA, Hodson L, Blaak EE, Wagenmakers AJ et al. Preferential uptake of dietary Fatty acids in adipose tissue and muscle in the postprandial period. Diabetes. 2007 Jan 1;56(1):168-176. https://doi.org/10.2337/db06-0822