@inbook{a75f656390124c818c659f7dd9ddcf68,
title = "Pre-analytical sample treatment and DNA extraction protocols for the detection of bacterial pathogens from whole blood",
abstract = "Molecular diagnostics is an increasing popular approach for the direct detection and identification of pathogenic bacteria in clinical samples. Conventional culture techniques are time-consuming and therefore causing a delay in the diagnosis of the patient. Alternative techniques based on nucleic acid amplification offer a shorter turn-around-time and the ability to identify fastidious and non-cultivable organisms. However, molecular detection of bacteria in blood, by for example PCR, RT-PCR, or sequencing of the 16S rDNA genes is often complicated by the presence of PCR-inhibitory compounds. Here we describe several different methods for the extraction of bacterial DNA from whole blood samples. The methods differ regarding costs, hands-on time as well as regarding sensitivity. In combination with a model PCR the detection limits that can be reached using the different methods range from 1,000 to 50 cfu/ml.",
author = "W.L.J. Hansen and C.A. Bruggeman and P.F. Wolffs",
year = "2013",
month = jan,
day = "1",
doi = "10.1007/978-1-60327-353-4_4",
language = "English",
isbn = "978-1-60327-352-7",
series = "Methods in Molecular Biology",
publisher = "Springer",
number = "943",
pages = "81--90",
editor = "M. Wilks",
booktitle = "PCR Detection of Microbial Pathogens",
address = "United States",
}