Platelet interaction with activated endothelium: mechanistic insights from microfluidics

Danielle M. Coenen, Tom G. Mastenbroek, Judith M. E. M. Cosemans*

*Corresponding author for this work

Research output: Contribution to journal(Systematic) Review article peer-review

75 Citations (Web of Science)
51 Downloads (Pure)

Abstract

Traditionally, in vitro flow chamber experiments and in vivo arterial thrombosis studies have been proved to be of vital importance to elucidate the mechanisms of platelet thrombus formation after vessel wall injury. In recent years, it has become clear that platelets also act as modulators of inflammatory processes, such as atherosclerosis. A key element herein is the complex cross talk between platelets, the coagulation system, leukocytes, and the activated endothelium. This review provides insight into the plateletendothelial interface, based on in vitro flow chamber studies and cross referenced with in vivo thrombosis studies. The main mechanisms of platelet interaction with the activated endothelium encompass (1) platelet rolling via interaction of platelet glycoprotein Ib-IX-V with endothelial-released von Willebrand factor with a supporting role for the P-selectin/P-selectin glycoprotein ligand 1 axis, followed by (2) firm platelet adhesion to the endothelium via interaction of platelet alpha(IIb)beta(3) with endothelial alpha(v)beta(3) and intercellular adhesion molecule 1, and (3) a stimulatory role for thrombin, the thrombospondin-1/CD36 axis and cyclooxygenase 1 in subsequent platelet activation and stable thrombus formation. In addition, the molecular mechanisms underlying the stimulatory effect of platelets on leukocyte transendothelial migration, a key mediator of atheroprogression, are discussed. Throughout the review, emphasis is placed on recommendations for setting up, reporting, interpreting, and comparing endothelial-lined flow chamber studies and suggestions for future studies.

Original languageEnglish
Pages (from-to)2819-2828
Number of pages10
JournalBlood
Volume130
Issue number26
DOIs
Publication statusPublished - 28 Dec 2017

Keywords

  • FLUID SHEAR-STRESS
  • ACUTE MYOCARDIAL-INFARCTION
  • IN-VITRO MICROVESSELS
  • THROMBUS FORMATION
  • P-SELECTIN
  • WHOLE-BLOOD
  • VITRONECTIN RECEPTOR
  • VASCULAR ENDOTHELIUM
  • INTEGRIN MAC-1
  • FLOW

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